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| Product Name | Enzymatic DNA Fragmentation Module (low-bias formulation) |
| Catalog No. | REKI-0102 |
| Description | A next-generation enzymatic fragmentation reagent for sequencing library preparation, built around a high-quality fragmentation enzyme with stable performance and low sequence bias. Replacing ultrasonication removes dedicated hardware and long handling from the workflow, and a 200 ng standard input fragments reproducibly for downstream end repair and adapter ligation, reducing both the time and cost of library construction. |
| Intended Use | DNA fragmentation before sequencing library preparation as a replacement for ultrasonic shearing. |
| Principle / Technology | Enzymatic cleavage shears DNA to library-relevant sizes with low sequence preference. |
| Sample Type | Genomic DNA |
| Performance Range / Specifications | Stable fragmentation with low bias at 200 ng standard input. |
| Specificity | Optimised formulation for reproducible smear distributions. |
| Reaction Conditions / Protocol | Incubate DNA with the fragmentation mix for the time giving the target size distribution. |
| Components / Formulation | Fragmentation enzyme premix. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 8 T, 24 T, 96 T, 5000 T |
| Product Form | Enzyme premix |
| Key Features | Sonication-free; low-bias shearing; bulk pack available. |
| Starting Material / Tissue Site | 200 ng standard input DNA |
| Readouts / Downstream Assays | Fragmented DNA for library construction |
For research use only, not for clinical use.
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