Enzymatic DNA Fragmentation Module (low-bias formulation)
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Enzymatic DNA Fragmentation Module (low-bias formulation)

Cat.No: REKI-0102 Datasheet

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Product Details Related Products
Product Name Enzymatic DNA Fragmentation Module (low-bias formulation)
Catalog No. REKI-0102
Description A next-generation enzymatic fragmentation reagent for sequencing library preparation, built around a high-quality fragmentation enzyme with stable performance and low sequence bias. Replacing ultrasonication removes dedicated hardware and long handling from the workflow, and a 200 ng standard input fragments reproducibly for downstream end repair and adapter ligation, reducing both the time and cost of library construction.
Intended Use DNA fragmentation before sequencing library preparation as a replacement for ultrasonic shearing.
Principle / Technology Enzymatic cleavage shears DNA to library-relevant sizes with low sequence preference.
Sample Type Genomic DNA
Performance Range / Specifications Stable fragmentation with low bias at 200 ng standard input.
Specificity Optimised formulation for reproducible smear distributions.
Reaction Conditions / Protocol Incubate DNA with the fragmentation mix for the time giving the target size distribution.
Components / Formulation Fragmentation enzyme premix.
Storage Conditions -25 to -15 °C
Shelf Life 18 months
Package Specifications 8 T, 24 T, 96 T, 5000 T
Product Form Enzyme premix
Key Features Sonication-free; low-bias shearing; bulk pack available.
Starting Material / Tissue Site 200 ng standard input DNA
Readouts / Downstream Assays Fragmented DNA for library construction

For research use only, not for clinical use.

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