- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Enzymatic DNA Fragmentation, End Repair and A-Tailing Module V2 |
| Catalog No. | REKI-0109 |
| Description | A second-generation enzymatic fragmentation reagent for sequencing library construction. A high-quality fragmentation enzyme cuts genomic DNA with stable, low-bias output across the full GC range, replacing time-consuming ultrasonic shearing with a benchtop reaction. The module integrates fragmentation, end repair and A-tailing, and accommodates 50 ng to 1 ug of genomic DNA from animal, plant or microbial sources. |
| Intended Use | Genomic DNA library construction for animal, plant and microbial samples; replacement of acoustic shearing in routine NGS workflows. |
| Principle / Technology | An engineered nuclease chops DNA under controlled conditions; the same tube then repairs fragment ends and adds the 3-prime A overhang needed for T-overhang adapter ligation. |
| Sample Type | Animal, plant, microbial genomic DNA |
| Performance Range / Specifications | Input 50 ng to 1 ug; enzymatic method; low fragmentation bias; consistent across all GC contents. |
| Reaction Conditions / Protocol | Incubate DNA with the fragmentation reagent under the recommended time-temperature programme, then proceed directly to adapter ligation. |
| Components / Formulation | Fragmentation enzyme, end repair and A-tailing mix, reaction buffers. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 24 T, 96 T |
| Product Form | Liquid reagents |
| Key Features | Single-tube fragmentation and end finishing; no shearing hardware required; reproducible size distributions across GC extremes. |
| Compatibility | Downstream adapter ligation modules on short-read sequencing platforms |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
