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| Product Name | EB Buffer, pH 7.2 |
| Catalog No. | RERE-0413 |
| Description | Extracting intact oocytes from surrounding tissue is gentle work, and EB buffer is built for it: 0.25 M sucrose cushions the cells osmotically while HEPES holds pH 7.2 and the chloride salts round out the ionic background. The preparation is tuned for oocyte isolation workflows where each wash step must neither swell nor shrink the delicate cells. Refrigerated storage preserves it for six months. |
| Intended Use | Isolation and extraction of oocytes from tissue in developmental and cell biology protocols. |
| Principle / Technology | Sucrose-supported osmotic balance with HEPES buffering protects oocytes through extraction washes. |
| Specificity | Composed of 0.25 M sucrose, sodium chloride, magnesium chloride and HEPES; pH 7.2. |
| Reaction Conditions / Protocol | Use as the isolation and wash medium in oocyte extraction procedures. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Osmotically cushioned extraction chemistry; HEPES buffered at pH 7.2; compact 100 mL format. |
For research use only, not for clinical use.
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