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| Product Name | E-64d, Membrane-Permeable Cysteine Protease Inhibitor |
| Catalog No. | RERE-0039 |
| Description | E-64d is a synthetic analogue of E-64, specifically the ethyl ester of E-64c, engineered to cross biological membranes. Once inside, it irreversibly inhibits thiol proteases including calpain and cysteine cathepsins F, K, B, H and L. The membrane-permeable design makes it the natural choice when intracellular protease activity must be blocked in living cells, complementing the non-permeable parent compound E-64 used in extract-level protection. |
| Intended Use | Blocking intracellular calpain and cysteine cathepsin activity in membrane-intact systems; protease control during protein extraction |
| Principle / Technology | Irreversible covalent inhibition of thiol protease active-site cysteine residues after membrane permeation |
| Detection Method | Calpain and cysteine cathepsins F, K, B, H and L |
| Sample Type | Living cells and membrane-intact systems; cell and tissue extracts |
| Performance Range / Specifications | Membrane permeation delivers inhibition to intracellular targets that the parent compound cannot reach |
| Specificity | Irreversible thiol protease inhibition with intracellular access |
| Reaction Conditions / Protocol | Dissolve in DMSO or ethanol at above 10 mg per mL and dose according to the application protocol |
| Components / Formulation | E-64d powder, 5 mg per vial |
| Storage Conditions | -20°C in dry conditions for at least 24 months |
| Shelf Life | At least 24 months |
| Package Specifications | 5 mg |
| Product Form | White powder |
| Quality Control | Purity at least 98% by TLC confirmed on the lot record |
| Key Features | Membrane-permeable ester design; irreversible thiol protease inhibition; complements non-permeable E-64; DMSO and ethanol soluble |
| Purity | At least 98% (TLC) |
| Concentration | Above 10 mg per mL in DMSO or ethanol; insoluble in water |
| Activity / Unit Definition | Irreversible thiol protease inhibition |
| Molecular Weight | 342.43 |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Wet ice |
| Expiration Date / Stability | At least 24 months dry at -20°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Combines with other protease class inhibitors for broad-coverage extraction and live-cell workflows |
| Application Notes / Precautions | Organic-solvent soluble only; keep DMSO carryover in working systems low; avoid skin contact |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Intracellular calpain and cathepsin inhibition, protected protein extraction from living systems |
For research use only, not for clinical use.
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