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| Product Name | Dual-Mode Total RNA Library Prep Kit for Mainstream High-Throughput Sequencing Platforms |
| Catalog No. | REKI-0070 |
| Description | A total RNA sequencing library prep kit compatible with both mainstream high-throughput sequencing platforms, containing RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific second-strand cDNA synthesis reagents, and library amplification reagents. It connects downstream of mRNA purification or rRNA depletion kits, and the two second-strand buffer options switch the workflow between non-stranded and stranded output via dUTP incorporation that a uracil-intolerant high-fidelity polymerase cannot amplify. |
| Intended Use | RNA-seq, lncRNA, circular RNA, RIP-seq, CLIP-seq, m6A, degraded-primer and ribosome profiling library construction from total RNA. |
| Principle / Technology | Fragmentation and reverse transcription precede second-strand synthesis with either standard dNTP or dUTP chemistry; end repair, A-tailing, adapter ligation and amplification complete the library. |
| Sample Type | Total RNA |
| Performance Range / Specifications | Fragment size tunable from roughly 250 to 500 bp by adjusting 94°C fragmentation time between 5 and 10 min |
| Specificity | Supports both strand-specific and non-stranded library modes |
| Storage Conditions | Store at -25 to -15°C |
| Package Specifications | 8 T / 24 T / 96 T |
| Product Form | Kit with modular buffer options |
| Key Features | Dual mainstream high-throughput sequencing compatibility; three-step merge of second-strand synthesis, end repair and A-tailing; dUTP strand-specific chemistry |
| Compatibility | Compatible with mainstream high-throughput sequencing platforms, mRNA capture kits and rRNA depletion kits |
For research use only, not for clinical use.
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