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| Product Name | Dual-Mode mRNA Library Prep Kit for mainstream sequencing Platforms (Stranded and Non-Stranded) |
| Catalog No. | REKI-0069 |
| Description | An mRNA transcriptome library prep kit developed for mainstream sequencing platforms that merges second-strand cDNA synthesis with end repair and A-tailing, cutting library prep time substantially. Two second-strand synthesis buffers let the user choose between conventional and strand-specific libraries. mRNA is isolated with oligo (dT) beads, then fragmented, reverse transcribed, end repaired, A-tailed, ligated and amplified into sequencing-ready libraries. |
| Intended Use | mRNA transcriptome library construction for eukaryotic total RNA on mainstream sequencing platforms. |
| Principle / Technology | Oligo (dT) bead capture isolates mRNA; fragmentation, first and second strand cDNA synthesis with end repair and A-tailing, adapter ligation and amplification build the library; a dUTP-based second-strand buffer delivers strand specificity. |
| Sample Type | Eukaryotic total RNA, 10 ng to 4 ug input |
| Storage Conditions | BOX I: 2 to 8°C; BOX II: -25 to -15°C |
| Package Specifications | 96 T / 1000 T |
| Product Form | Two-box kit with oligo (dT) magnetic beads and library modules |
| Key Features | Second-strand synthesis merged into end repair step; switchable stranded or non-stranded chemistry; scalable to 1000 T |
| Compatibility | Compatible with mainstream sequencing high-throughput sequencing platforms |
For research use only, not for clinical use.
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