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| Product Name | Dried Blood Spot Preservation Buffer, Extraction Ready |
| Catalog No. | ATR-SPS-0002 |
| Description | A formulated extraction and preservation buffer for the elution of proteins, metabolites, and nucleic acids from dried blood spot cards used in newborn screening and pharmacokinetic studies. |
| Intended Use | Elution and stabilization of analytes from dried blood spot (DBS) filter paper cards for subsequent analysis by LC-MS, immunoassay, and PCR-based methods. |
| Principle / Technology | Detergent and protein components in the buffer solubilize dried blood components and stabilize eluted analytes; EDTA chelates metals that catalyze analyte degradation. |
| Detection Method | Analyte recovery from DBS punches, stability over 7 days at 4°C versus baseline |
| Sample Type | Dried blood spots on Whatman 903 or equivalent filter paper cards |
| Performance Range / Specifications | >85% recovery for amino acids, acylcarnitines, and steroids; stability up to 7 days at 4°C |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Punch 3.2 mm DBS disk into 100 µL buffer; shake at 600 rpm for 30 min at room temperature; centrifuge; use supernatant for analysis. |
| Components / Formulation | PBS pH 7.4, 0.1% BSA, 0.05% Tween 20, 1 mM EDTA, 0.02% sodium azide |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 50 mL, 100 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Analyte recovery panel testing, pH, sterility |
| Key Features | Optimized for DBS extraction; ready to use; preservative included; validated for newborn screening analytes |
For research use only, not for clinical use.
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