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| Product Name | DNA End Repair and dA-Tailing Module for Nanopore Sequencing |
| Catalog No. | REKI-0116 |
| Description | An end-repair and A-tailing module engineered specifically for nanopore long-read library construction. Directed optimisation of the enzymes and buffer lets the module repair 100 ng to 1.5 ug of genomic DNA or amplicon material efficiently, and the end-prepared product carries a 5-prime phosphate and 3-prime dA overhang that feeds straight into barcode ligation without intermediate purification, shortening the path to sequencer-ready libraries. |
| Intended Use | End repair and A-tailing ahead of barcode ligation in nanopore library construction, gDNA and amplicon library workflows, long-read sequencing sample preparation. |
| Principle / Technology | Repair enzymes polish damaged or overhang-bearing DNA ends while a polymerase adds the dA overhang required by downstream ligation chemistry. |
| Sample Type | Genomic DNA, PCR amplicons |
| Performance Range / Specifications | Input 100 ng to 1.5 ug; efficient repair across the range; purification-free transition to barcode ligation. |
| Reaction Conditions / Protocol | Incubate DNA with the end repair and dA-tailing mix, then proceed directly to barcode adapter ligation. |
| Components / Formulation | End repair enzyme mix, dA-tailing polymerase, reaction buffer. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Liquid reagents |
| Key Features | No cleanup between end repair and barcode ligation; tuned for nanopore adapter chemistry; broad input window. |
| Compatibility | Nanopore (ONT) sequencing platform; pairs with companion barcode and adapter ligation modules |
For research use only, not for clinical use.
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