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| Product Name | DNA Adapter Ligation Module with High-Efficiency Ligase (standard packs) |
| Catalog No. | REKI-0092 |
| Description | A ligation module for sequencing library construction on dual short-read platforms, powered by a proprietary high-efficiency T4-type ligase. The enzyme joins adapters to blunt-ended or 3-prime dA-tailed fragments with strong efficiency across all sample classes, and shows particular advantage on complex or structured nucleic acid fragments where ordinary ligases stall. Sequencing on both platforms has validated library quality, and the module suits automated workflows. |
| Intended Use | Adapter ligation in DNA library construction for dual-platform sequencing; handling of complex structured fragments. |
| Principle / Technology | A proprietary high-efficiency T4-type ligase joins adapters to blunt or dA-tailed double-stranded fragments. |
| Sample Type | Blunt-ended or 3-prime dA-tailed DNA fragments |
| Performance Range / Specifications | High ligation efficiency across sample types; sequencing-validated on both platforms; automation compatible. |
| Specificity | Proprietary ligase formulation with advantage on structured fragments. |
| Reaction Conditions / Protocol | Combine end-repaired DNA with adapters and the ligation mix, incubate, then purify libraries. |
| Components / Formulation | Ligase and ligation buffer module. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 24 T, 96 T |
| Product Form | Enzyme and buffer module |
| Key Features | Dual-platform validated; strong on difficult fragments; automation ready. |
| Compatibility | Dual short-read sequencing platforms; automation workflows |
| Readouts / Downstream Assays | Adapter-ligated sequencing libraries |
For research use only, not for clinical use.
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