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| Product Name | Deaminase-Based Transcription Factor Footprinting Library Preparation Kit for Illumina |
| Catalog No. | NG-QCY-0028 |
| Description | A deaminase-based library preparation workflow for mapping transcription factor footprints and accessible chromatin at near single-base resolution. |
| Intended Use | Epigenetic profiling, chromatin accessibility analysis and global transcription factor footprinting. |
| Principle / Technology | A double-stranded DNA cytosine deaminase converts exposed cytosines to uracil while protein-bound DNA is protected, allowing sequencing-based identification of occupied sites. |
| Detection Method | Illumina next-generation sequencing with cytosine-conversion and footprinting analysis. |
| Sample Type | Isolated cells; performance was demonstrated with 5,000, 50,000 and 100,000-cell inputs. |
| Performance Range / Specifications | One-day library preparation; near single-base resolution; 12 or 48 reactions. |
| Sensitivity / LOD | Demonstrated with cell inputs as low as 5,000 cells. |
| Specificity | Protein-protected DNA remains relatively unmodified, enabling localized identification of transcription factor binding footprints. |
| Reaction Conditions / Protocol | Prepare nuclei or permeabilized cells, perform controlled deaminase treatment, complete library construction and sequence using validated depth and conversion-quality thresholds. |
| Components / Formulation | Deaminase footprinting reagents, permeabilization reagents and Illumina-compatible library preparation components supplied in frozen and refrigerated modules. |
| Storage Conditions | Store the digitonin-containing and other frozen reagents at −30 to −15°C; store the refrigerated module at 2–8°C. |
| Package Specifications | 12 reactions; 48 reactions. |
| Product Form | Multi-module epigenomic library preparation kit. |
| Key Features | Global transcription factor mapping; near single-base resolution; one-day workflow; low cell input; simultaneous accessibility and footprint information. |
| Shipping Conditions | Ship frozen reagents at or below 0°C and refrigerated reagents with ice packs. |
| Expiration Date / Stability | The supplied 1% digitonin solution is reported stable for approximately 1 week at 15–25°C; other components require their specified storage temperatures. |
| Compatibility | Compatible with Illumina sequencing platforms. |
| Application Notes / Precautions | An initial low-depth sequencing quality check may be used before deeper sequencing. Conversion rate, TSS signal and sequencing depth should be validated for each sample type. |
For research use only, not for clinical use.
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