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| Product Name | DAPI Antifade Aqueous Mounting Medium (Em 455 nm) |
| Catalog No. | RERE-0055 |
| Description | This water based mounting medium carries the blue nuclear fluorescent probe DAPI, emitting at 455 nm, so nuclear staining and coverslip mounting complete in a single step. It markedly slows the photobleaching of common fluorophores, and after drying it forms a firm, optically transparent coating suited to semi permanent slide preservation. The medium fits cell preparations and tissue sections in immunofluorescence, immunocytochemistry and immunohistochemistry workflows whose final staining step ran in aqueous media. |
| Intended Use | Combined nuclear staining and mounting of cell preparations and tissue sections for immunofluorescence, immunocytochemistry and immunohistochemistry. |
| Principle / Technology | DAPI intercalates into nuclear DNA and fluoresces blue at 455 nm, while antifade additives slow fluorophore photobleaching during observation. |
| Performance Range / Specifications | Simultaneous nuclear counterstain and mount; semi permanent, optically transparent seal. |
| Specificity | Nuclear specific blue fluorescence with 455 nm emission. |
| Reaction Conditions / Protocol | Apply the medium to the stained specimen, lower the coverslip and allow the coating to dry; observe nuclei under DAPI channels together with target fluorophores. |
| Storage Conditions | Room temperature protected from light |
| Package Specifications | 4 mL; 20 mL |
| Product Form | Aqueous mounting medium containing DAPI |
| Key Features | One step nuclear staining and mounting; marked antifade effect; firm transparent dried coating. |
| Shipping Conditions | Room temperature |
| Regulatory / Compliance | For research use. |
| Batch-to-Batch Consistency | Lot controlled manufacture. |
For research use only, not for clinical use.
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