- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Crosslinking Reagent for Chromatin Immunoprecipitation, 16% Formaldehyde, Methanol-Free |
| Catalog No. | ATR-SPS-0025 |
| Description | Methanol-free 16% formaldehyde in sealed glass ampules under argon for ChIP and protein-DNA crosslinking applications. Methanol-free formulation eliminates protein precipitation artifacts. Ampule format ensures fresh, unoxidized reagent for every experiment, avoiding formic acid formation that compromises crosslinking efficiency. |
| Intended Use | ChIP/ChIP-seq protein-DNA crosslinking; 3C/4C/Hi-C chromosome conformation capture; cell/tissue fixation for IHC; reversible crosslinking in protein interaction studies; CLIP-seq RNA-protein crosslinking. |
| Principle / Technology | Formaldehyde crosslinks primary amines (Lys) and thiols (Cys) forming reversible methylene bridges; crosslinks reversed by heat (65 °C) with high salt or proteinase K |
| Detection Method | For ChIP: add to medium to 1% final; incubate 5-15 min RT; quench with 125 mM glycine 5 min; wash with cold PBS |
| Sample Type | Adherent and suspension cells; fresh/frozen tissue; 10⁶-10⁸ cells per ChIP |
| Reaction Conditions / Protocol | Crosslinking: 5-15 min RT (optimize per cell type/target); quenching: 5 min glycine; over-fixation impairs shearing and epitope accessibility |
| Components / Formulation | Formaldehyde 16% (w/v) in water, methanol-free (<1%); 10×1 mL ampules; supplied under argon |
| Storage Conditions | 15-25 °C; protect from light; store ampules upright; do not freeze; single-use |
| Shelf Life | 24 months in sealed ampule |
| Package Specifications | 10×1 mL, 20×1 mL, 50×1 mL ampules |
| Product Form | Colorless liquid in sealed glass ampule under inert gas |
| Key Features | Methanol-free <1%; sealed under argon; ampule format prevents oxidation; consistent 16% by titrimetry; validated for ChIP-seq/Hi-C |
| Purity | Formaldehyde 16.0±0.5% (w/v); methanol <1.0%; formic acid <0.05%; ACS grade |
| Concentration | 16% (w/v); working dilution 1:16 to 1% |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | pH 3.5-4.5 (neat) |
| Shipping Conditions | Ambient; flammable liquid; protect from light; do not freeze |
| Expiration Date / Stability | 24 months in sealed ampule; use within 5 min after opening; discard opened ampule |
| Regulatory / Compliance | For laboratory and research use only; RUO; formaldehyde toxic/carcinogenic — fume hood required; CAS 50-00-0; GHS06/GHS08 |
| Compatibility | Compatible with DMEM/RPMI; glycine quenching essential — do not use Tris; avoid amino-containing buffers for pre-fixation washes |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Optimize crosslinking time: histones 5-10 min, TFs 10-15 min, co-factors 15-20 min. Over-fixation >20 min causes shearing resistance. Always quench with glycine excess. For Hi-C: 10-30 min per protocol. |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
