- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | CRISPR Guide RNA (sgRNA) Synthesis Service, In Vitro Transcription Template |
| Catalog No. | PRIPRO-0031 |
| Description | Custom synthesis of single-guide RNA (sgRNA) in vitro transcription templates as linear dsDNA (gBlocks) for CRISPR-Cas9 gene editing applications. Each template contains a T7 promoter, target-specific 20-nt guide sequence, and the conserved sgRNA scaffold sequence. Templates are supplied as purified dsDNA fragments ready for in vitro transcription using T7 RNA polymerase, producing functional sgRNA for Cas9 nuclease delivery. |
| Intended Use | CRISPR-Cas9 sgRNA in vitro transcription; gene knockout generation; gene editing validation; CRISPR library construction; transgenic model organism generation; therapeutic gene editing research. |
| Principle / Technology | Synthetic dsDNA template contains T7 promoter + 20-nt target-specific sequence (protospacer) + conserved sgRNA scaffold; T7 RNA polymerase in vitro transcription produces ~100 nt sgRNA; sgRNA complexes with Cas9 protein and directs nuclease activity to complementary genomic target sites adjacent to PAM (NGG for SpCas9) |
| Detection Method | Customer provides 20-nt target sequence (protospacer) and specifies Cas9 variant (SpCas9, SaCas9, etc.); template synthesized with appropriate scaffold sequence; delivered as dsDNA (500 ng-5 µg); in vitro transcription performed by customer using T7 RNA polymerase |
| Sample Type | Customer provides 20-nt guide sequence; indicate PAM sequence (NGG for SpCas9) and whether target is on sense or antisense strand; specify Cas9 variant for appropriate scaffold |
| Components / Formulation | Linear dsDNA template containing T7 promoter + guide sequence + sgRNA scaffold; synthesis scale 500 ng, 2 µg, 5 µg; concentration normalized to 50 ng/µL in TE buffer |
| Storage Conditions | -20 °C; avoid repeated freeze-thaw cycles; dsDNA stable at -20 °C |
| Shelf Life | 24 months at -20 °C |
| Package Specifications | Custom service; 500 ng, 2 µg, 5 µg per template; multiple templates available for multiplex editing |
| Product Form | Purified linear dsDNA in TE buffer (pH 8.0), 50 ng/µL; quality control by capillary electrophoresis |
| Key Features | Custom sgRNA template design; compatible with standard T7 in vitro transcription; validated scaffold sequences for SpCas9, SaCas9, and other variants; high-yield transcription producing 50-100 µg sgRNA per 1 µg template; purified dsDNA verified by Bioanalyzer/TapeStation; typical delivery 5-7 business days |
| Purity | >90% full-length dsDNA by capillary electrophoresis; endotoxin <0.1 EU/µg; sequence verified by Sanger sequencing |
| Concentration | As specified in synthesis report; typically lyophilized or at 100 µM in TE |
| Activity / Unit Definition | Primer efficiency 90–110% in qPCR; Tm within ±2 °C of calculated value |
| Molecular Weight | As specified per MALDI-TOF certificate; within 0.1% of theoretical mass |
| Source / Origin | Solid-phase phosphoramidite chemical synthesis |
| pH Range / Optimal pH | Stable in TE buffer pH 7.0–8.0; avoid acidic conditions causing depurination |
| Shipping Conditions | Ambient temperature; dsDNA stable at RT for 1 week during transit; long-term store at -20 °C |
| Expiration Date / Stability | 24 months at -20 °C; avoid >5 freeze-thaw cycles; aliquot upon receipt |
| Regulatory / Compliance | For laboratory and research use only; RUO; CRISPR-Cas9 may be subject to intellectual property restrictions — verify licensing for commercial use; ISO 9001 |
| Compatibility | Compatible with standard PCR, qPCR, and sequencing platforms |
| Recommended Buffer System | Resuspend in nuclease-free water or TE buffer pH 8.0 |
| Application Notes / Precautions | Protect fluorescent probes from light; avoid repeated freeze-thaw cycles; aliquot for single use |
| Batch-to-Batch Consistency | MALDI-TOF mass confirmation per lot; qPCR performance within 0.5 Ct of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
