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| Product Name | Fluorescence In Situ Hybridization (FISH) Probe Synthesis Service, Custom Locus-Specific |
| Catalog No. | PRIPRO-0032 |
| Description | Custom synthesis of locus-specific FISH probes for detection of chromosomal abnormalities, gene amplifications, deletions, and rearrangements by fluorescence microscopy. Probes are generated from BAC (Bacterial Artificial Chromosome) clones or long-range PCR products, fluorescently labeled with SpectrumGreen, SpectrumOrange, SpectrumRed, or SpectrumAqua fluorophores via nick translation. Each probe includes hybridization buffer for direct use. |
| Intended Use | Detection of gene amplification (HER2, EGFR, MYCN, MET); chromosomal translocation detection (BCR-ABL, EML4-ALK, TMPRSS2-ERG); gene deletion detection (PTEN, CDKN2A, TP53); chromosomal enumeration for aneuploidy screening; break-apart and dual-fusion probe designs. |
| Principle / Technology | BAC DNA or long-range PCR product is labeled with fluorescent-dUTP by nick translation; labeled probe DNA is ethanol-precipitated with Cot-1 DNA (blocks repetitive sequences) and resuspended in hybridization buffer; probe hybridizes to complementary chromosomal target on metaphase spreads or interphase nuclei; fluorescence microscopy visualizes signal at target locus |
| Detection Method | Customer provides target genomic coordinates or gene name; probes designed from BAC clones spanning 100-300 kb at target locus; labeled with specified fluorophore; supplied as 200 µL hybridization-ready probe (20 tests at 10 µL/test) |
| Sample Type | Customer provides gene name or genomic coordinates (hg19/hg38); specify desired fluorophore (Green, Orange, Red, Aqua); indicate probe design type: locus-specific, break-apart (translocation), or dual-fusion |
| Components / Formulation | Fluorescently labeled probe DNA in hybridization buffer (formamide, dextran sulfate, SSC); Cot-1 blocking DNA included; probe volume 200 µL (20 tests); DAPI counterstain optional |
| Storage Conditions | -20 °C; protect from light; hybridization buffer contains formamide — do not expose to air unnecessarily |
| Shelf Life | 12 months at -20 °C protected from light |
| Package Specifications | Custom service; 200 µL probe (20 tests), 500 µL (50 tests); each test 10 µL |
| Product Form | Ready-to-use fluorescent probe in hybridization buffer; supplied in amber tube |
| Key Features | BAC clone-based probes (100-300 kb coverage); multiple fluorophore options (Green, Orange, Red, Aqua); hybridization buffer included for direct use; nick translation labeling for uniform probe labeling; 20 tests per 200 µL; validated on normal metaphase controls; FISH protocol provided |
| Purity | Probe concentration 5-10 ng/µL; labeling efficiency >80% by spectrophotometry; probe size 200-600 bp after nick translation (optimized for tissue penetration); human Cot-1 DNA included for repeat suppression |
| Concentration | As specified in synthesis report; typically lyophilized or at 100 µM in TE |
| Activity / Unit Definition | Primer efficiency 90–110% in qPCR; Tm within ±2 °C of calculated value |
| Molecular Weight | As specified per MALDI-TOF certificate; within 0.1% of theoretical mass |
| Source / Origin | Solid-phase phosphoramidite chemical synthesis |
| pH Range / Optimal pH | Stable in TE buffer pH 7.0–8.0; avoid acidic conditions causing depurination |
| Shipping Conditions | Cold pack -20 °C; protect from light; do not allow to warm above 4 °C during transit |
| Expiration Date / Stability | 12 months at -20 °C protected from light; avoid repeated freeze-thaw; discard if probe signal intensity decreases >50% vs. new lot |
| Regulatory / Compliance | For laboratory and research use only; RUO; for clinical diagnostic use, additional validation required; formamide is a reproductive toxin — handle per institutional safety guidelines; ISO 9001 |
| Compatibility | Compatible with standard PCR, qPCR, and sequencing platforms |
| Recommended Buffer System | Resuspend in nuclease-free water or TE buffer pH 8.0 |
| Application Notes / Precautions | Protect fluorescent probes from light; avoid repeated freeze-thaw cycles; aliquot for single use |
| Batch-to-Batch Consistency | MALDI-TOF mass confirmation per lot; qPCR performance within 0.5 Ct of reference lot |
For research use only, not for clinical use.
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