- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | 5′-TET (Tetrachlorofluorescein) Labeled Oligonucleotide |
| Catalog No. | PRIPRO-0007 |
| Description | A custom DNA oligonucleotide carrying a 5′-terminal tetrachlorofluorescein (TET) fluorophore. TET emits yellow-green fluorescence with excitation at 521 nm and emission at 538 nm, positioned spectrally between FAM and HEX. This intermediate wavelength makes TET an excellent choice for three-color multiplex detection schemes and as an internal standard in fragment analysis. The dye is attached through a phosphoramidite linker during automated synthesis on the 5′-hydroxyl group of the terminal nucleotide. HPLC purification removes unincorporated dye and truncated sequences to ensure a homogeneous fluorescent product. |
| Intended Use | Intended for three-color multiplex fragment analysis, SNP genotyping by fluorescent PCR-CE, gene expression profiling with multiplex qPCR panels, and as a donor fluorophore in fluorescence resonance energy transfer (FRET) assays. |
| Principle / Technology | Solid-phase oligonucleotide synthesis with 5′-TET phosphoramidite; HPLC purification under dark conditions |
| Detection Method | UV-Vis spectrophotometry (A260 and A521); fluorescence spectroscopy; HPLC-fluorescence analysis |
| Sample Type | Genomic DNA, cDNA, or PCR products for fluorescent detection applications |
| Performance Range / Specifications | 10–60 bases; TET labeling efficiency ≥85% by HPLC peak area ratio |
| Sensitivity / LOD | Detection to sub-nanomolar level on CE and plate reader platforms |
| Specificity | Spectral separation from FAM and HEX allows three-color multiplex without significant cross-talk after color compensation |
| Reaction Conditions / Protocol | Dissolve in nuclease-free water or TE buffer pH 8.0 to 100 µM. Protect from ambient light. Use at 0.1–0.5 µM for single-color detection; adjust for multiplex panels. |
| Components / Formulation | Light-protected vial containing lyophilized TET-oligonucleotide |
| Storage Conditions | –20 °C protected from light; use amber tubes for solution storage |
| Shelf Life | 12 months at –20 °C dry (dark); 6 months at –20 °C in dark solution |
| Package Specifications | Amber tube with 5–50 nmol yield; COA with spectral data |
| Product Form | Lyophilized pale yellow to greenish pellet |
| Quality Control | HPLC purity ≥90%; MALDI-TOF MS; UV-Vis scan (220–700 nm); dye per oligo ratio from A521/A260; fluorescence emission scan with peak verification at 538 ± 5 nm. |
| Key Features | Intermediate spectral position for three-color multiplexing; compatible with existing FAM/HEX filter sets; HPLC purified for uniform dye-labeling; suitable for FRET donor applications with TAMRA or BHQ acceptors. |
| Purity | ≥90% by HPLC (fluorescence detection); homogeneous dye-labeled population |
| Concentration | Resuspend to 100 µM based on extinction coefficient; scale-dependent yield |
| Activity / Unit Definition | Extinction coefficient ~72,000 M⁻¹cm⁻¹ at 521 nm; emission maximum at 538 nm in TE buffer pH 8.0 |
| Molecular Weight | Oligo molecular weight plus approximately 556 Da for TET linker moiety |
| Source / Origin | Synthetic oligonucleotide; TET fluorophore from chemical synthesis |
| pH Range / Optimal pH | Fluorescence optimal at pH 7.5–9.0; signal decreases in acidic conditions |
| Shipping Conditions | Ambient temperature; lyophilized in foil-wrapped or amber tube |
| Expiration Date / Stability | 12 months at –20 °C dry; 6 months in solution at –20 °C (dark) |
| Regulatory / Compliance | For research use only; RUO product |
| Compatibility | TET channel detection on ABI 310/3100/3730 series; compatible with Bio-Rad and Qiagen qPCR instruments having TET/VIC channels; FRET compatible with TAMRA quenchers |
| Recommended Buffer System | TE buffer pH 8.0 or nuclease-free water; avoid acidic pH for consistent fluorescence |
| Application Notes / Precautions | For three-color multiplex, perform color calibration matrix with single-dye samples before running multiplexed panels. Keep working solutions on ice and protected from light during use. |
| Batch-to-Batch Consistency | Fluorescence emission peak position maintained within ±3 nm; dye incorporation efficiency consistent within ±10% across lots |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
