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| Product Name | Custom DNA Oligonucleotide Synthesis Service (25 nmol, Desalted) |
| Catalog No. | PRIPRO-0001 |
| Description | A custom DNA oligonucleotide synthesis service delivering unmodified single-stranded DNA fragments at the 25 nanomole synthesis scale with standard desalting purification. Sequences are built via solid-phase phosphoramidite chemistry on automated synthesizers using controlled pore glass (CPG) solid supports. Each oligo is cleaved, deprotected, and desalted to remove residual small-molecule by-products. The final product is supplied as a dried pellet quantified by UV absorbance at 260 nm and accompanied by a certificate of analysis. Suitable for routine PCR, sequencing, site-directed mutagenesis, and general molecular biology applications where standard purity is acceptable. |
| Intended Use | Designed for use as primers in standard endpoint PCR, Sanger sequencing reactions, site-directed mutagenesis, and general molecular cloning workflows in research laboratories. |
| Principle / Technology | Solid-phase phosphoramidite synthesis with CPG support |
| Detection Method | UV spectrophotometry (A260) for quantification and purity assessment |
| Sample Type | Purified template DNA or cDNA for downstream PCR/sequencing |
| Performance Range / Specifications | 2–60 bases; synthesis yield decreases with length >50 nt |
| Sensitivity / LOD | N/A — this is a custom synthesis reagent, not an assay |
| Specificity | Sequence-specific as ordered; no cross-hybridization guarantee |
| Reaction Conditions / Protocol | Resuspend in nuclease-free water or TE buffer (pH 8.0) to desired working concentration (typically 100 µM stock). Use 0.1–1.0 µM final concentration per primer in PCR. |
| Components / Formulation | One tube containing lyophilized oligonucleotide pellet |
| Storage Conditions | –20 °C, protected from light; avoid repeated freeze-thaw cycles |
| Shelf Life | 24 months when stored as dried pellet at –20 °C |
| Package Specifications | One microcentrifuge tube (1.5 mL or 2.0 mL) with 25 nmol scale yield |
| Product Form | Lyophilized white to off-white pellet |
| Quality Control | Each oligo is analyzed by UV spectrophotometry (A260/A280 ratio ≥1.8) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for molecular weight confirmation. A certificate of analysis is provided for each synthesis lot. |
| Key Features | Rapid turnaround within 2–3 business days; online sequence entry and order tracking; customizable 5′ or 3′ modifications available; competitive pricing for routine research oligos; re-synthesis guarantee for quality failures. |
| Purity | ≥85% by capillary electrophoresis; desalted grade |
| Concentration | Lyophilized from a nominal 25 nmol synthesis scale; typical yield 6–15 OD260 units (sequence dependent) |
| Activity / Unit Definition | Hybridizes to complementary target sequence under standard annealing conditions |
| Molecular Weight | Calculated based on sequence composition; reported on COA |
| Source / Origin | Synthetic; no animal- or plant-derived materials |
| pH Range / Optimal pH | Optimal resuspension in TE buffer pH 7.5–8.5 |
| Shipping Conditions | Ambient temperature; lyophilized oligos are stable during transit |
| Expiration Date / Stability | 24 months at –20 °C dry; 12 months at 2–8 °C in solution |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic procedures |
| Compatibility | Compatible with standard Taq DNA polymerase, proofreading polymerases, and all major thermal cycler brands |
| Recommended Buffer System | TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) recommended for resuspension |
| Application Notes / Precautions | Centrifuge tube briefly before opening to ensure pellet is at bottom. Resuspend in sterile nuclease-free water or TE buffer. Verify concentration by UV measurement before use. For long-term storage, aliquot and keep at –20 °C. |
| Batch-to-Batch Consistency | Each synthesis batch is independently verified by MS and UV; mass accuracy within ±0.05% of theoretical value |
For research use only, not for clinical use.
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