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| Product Name | 3′-TAMRA (Carboxytetramethylrhodamine) Labeled Oligonucleotide |
| Catalog No. | PRIPRO-0009 |
| Description | A custom DNA oligonucleotide with a 3′-terminal carboxytetramethylrhodamine (TAMRA) fluorescent label. TAMRA is one of the most widely used orange-red fluorophores, exhibiting excitation at approximately 555 nm and emission at 580 nm. The 3′-position labeling is achieved by starting synthesis on a TAMRA-derivatized controlled pore glass (CPG) solid support, ensuring that every full-length oligo carries the dye at the 3′ end. This configuration leaves the 5′ end free for additional modifications, enzymatic reactions, or primer extension. TAMRA also functions as a FRET acceptor when paired with FAM, TET, or HEX donors in dual-labeled probe systems. HPLC purification removes non-labeled truncated sequences, guaranteeing a homogeneous labeled product. |
| Intended Use | Designed for 3′-end fluorescent detection in fragment analysis, as a FRET acceptor in dual-labeled probes, fluorescent primer extension assays, 3′-labeled antisense tracking in cell-based studies, and molecular beacon construction with terminal fluorophore. |
| Principle / Technology | Synthesis initiated from TAMRA-CPG solid support; all full-length chains carry 3′-TAMRA; HPLC purification isolates dye-labeled full-length oligo |
| Detection Method | UV-Vis (A260, A555); fluorescence spectroscopy (Ex 555 / Em 580 nm); HPLC-fluorescence; MALDI-TOF MS |
| Sample Type | PCR products for 3′-label detection; cell lysates for uptake studies; complementary target for FRET probe hybridization |
| Performance Range / Specifications | 10–50 bases optimal for 3′-CPG synthesis; longer sequences available |
| Sensitivity / LOD | Detectable at 0.5–2 nM in standard fluorescence readers and CE |
| Specificity | 3′-TAMRA on every full-length chain; truncated sequences lack dye and are removed by HPLC |
| Reaction Conditions / Protocol | Resuspend in nuclease-free water at 100 µM stock. Use in FRET probe pairs with FAM/TET/HEX donors at 1:1 to 1:2 donor-to-acceptor ratio. Protect from light. |
| Components / Formulation | Light-protected tube with lyophilized 3′-TAMRA oligo; COA |
| Storage Conditions | –20 °C in dark container; desiccated for long-term storage |
| Shelf Life | 12 months dry at –20 °C (dark); 6 months at –20 °C in solution (dark) |
| Package Specifications | Amber tube with 5–50 nmol scale; foil outer wrap |
| Product Form | Lyophilized pink to magenta-red pellet |
| Quality Control | HPLC purity ≥90%; MALDI-TOF MS; A555/A260 ratio confirming 3′ dye incorporation; residual CPG-derived contaminants below limit; fluorescence emission spectrum verified. |
| Key Features | 3′-labeling leaves 5′ end free for further modification; CPG-based labeling guarantees dye on every full-length chain; classic FRET acceptor for FAM, TET, HEX; HPLC purified; suitable for molecular beacon construction. |
| Purity | ≥90% by HPLC; 3′-dye incorporation >95% by peak area at 555 nm |
| Concentration | Resuspend to 100 µM based on extinction coefficient; scale-dependent yield |
| Activity / Unit Definition | Extinction coefficient ~65,000 M⁻¹cm⁻¹ at 555 nm; emission maximum 580 nm; effective FRET acceptor with R0 ~50 Å for FAM-TAMRA pair |
| Molecular Weight | Oligonucleotide MW plus approximately 470 Da for 3′-TAMRA CPG linker moiety |
| Source / Origin | Synthetic; TAMRA-CPG solid support from chemical synthesis |
| pH Range / Optimal pH | Fluorescence stable pH 6.0–9.0; minimal pH sensitivity |
| Shipping Conditions | Ambient temperature in foil-wrapped packaging |
| Expiration Date / Stability | 12 months dry at –20 °C (dark); 6 months in TE at –20 °C (dark) |
| Regulatory / Compliance | For research use only; RUO product |
| Compatibility | FRET compatible with FAM, TET, HEX, and JOE donors; detection on TAMRA/NED channel of ABI capillary sequencers; compatible with Bio-Rad and Roche qPCR platforms |
| Recommended Buffer System | TE buffer pH 8.0 or nuclease-free water; TAMRA fluorescence broadly pH-independent |
| Application Notes / Precautions | Verify FRET efficiency by measuring donor quenching upon hybridization with complementary target. For molecular beacon construction, pair with a 5′ quencher (BHQ or DABCYL) for optimal signal-to-noise ratio. |
| Batch-to-Batch Consistency | Dye incorporation confirmed by A555/A260 ratio; fluorescence emission peak within ±3 nm; HPLC purity within ±3% across lots |
For research use only, not for clinical use.
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