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| Product Name | Locked Nucleic Acid (LNA)-Enhanced qPCR Probe Synthesis Service |
| Catalog No. | PRIPRO-0027 |
| Description | A custom probe synthesis service incorporating locked nucleic acid nucleotides into hydrolysis probe sequences for enhanced thermal stability and target discrimination. LNA modifications contain a methylene bridge locking the ribose ring into a C3′-endo conformation, pre-organizing the base for hybridization and increasing the melting temperature by 2–8 °C per LNA substitution. LNA-enhanced probes enable shorter probe designs for discriminating single nucleotide polymorphisms and mutations. |
| Intended Use | High-specificity qPCR target detection with enhanced single nucleotide discrimination using LNA-modified hydrolysis probes. |
| Principle / Technology | LNA-mediated probe duplex stabilization enabling short probe designs with high melting temperatures for allele discrimination |
| Detection Method | Real-time fluorescence detection on qPCR platforms |
| Sample Type | Genomic DNA samples for SNP genotyping, somatic mutation detection, and pathogen strain identification |
| Performance Range / Specifications | Probe length: 8–15 nucleotides with 2–6 LNA substitutions; dual-labeled with FAM/BHQ1, HEX/BHQ1, or CY5/BHQ2; purification: HPLC |
| Sensitivity / LOD | Allele discrimination ΔCt ≥10 cycles between matched and mismatched templates |
| Specificity | Single LNA substitution at SNP site increases mismatch discrimination by ≥5 °C compared to DNA-only probe |
| Reaction Conditions / Protocol | Design probe with LNA nucleotide at the polymorphic site; Tm calculation requires LNA-adjusted thermodynamic parameters; HPLC purification removes unincorporated dye |
| Components / Formulation | LNA-modified oligonucleotide probe, HPLC purified, dual-labeled with 5′-fluorophore and 3′-quencher; MALDI-TOF mass confirmation and HPLC purity certificate provided |
| Storage Conditions | Lyophilized: –20 °C, protected from light; reconstituted: –20 °C, single-use aliquots |
| Shelf Life | 24 months lyophilized at –20 °C |
| Package Specifications | Per probe: 5 nmol or 20 nmol synthesis scale |
| Product Form | Lyophilized solid, colored (fluorophore-dependent) |
| Quality Control | MALDI-TOF mass within specification; HPLC purity ≥90%; fluorescence quenching efficiency ≥95% |
| Key Features | LNA incorporation enables functional probe designs of 8–12 nucleotides that are impossible with standard DNA chemistry |
| Purity | HPLC purification ≥90% full-length product; desalted ≥85% |
| Concentration | As specified in synthesis report; typically lyophilized or at 100 µM in TE |
| Activity / Unit Definition | Primer efficiency 90–110% in qPCR; Tm within ±2 °C of calculated value |
| Molecular Weight | As specified per MALDI-TOF certificate; within 0.1% of theoretical mass |
| Source / Origin | Solid-phase phosphoramidite chemical synthesis |
| pH Range / Optimal pH | Stable in TE buffer pH 7.0–8.0; avoid acidic conditions causing depurination |
| Shipping Conditions | Ambient temperature for lyophilized oligos; cold packs for fluorescent probes |
| Expiration Date / Stability | 24 months lyophilized at –20 °C; 6 months reconstituted at –20 °C |
| Regulatory / Compliance | Research use as standard; GMP-grade with full documentation available |
| Compatibility | Compatible with standard PCR, qPCR, and sequencing platforms |
| Recommended Buffer System | Resuspend in nuclease-free water or TE buffer pH 8.0 |
| Application Notes / Precautions | Protect fluorescent probes from light; avoid repeated freeze-thaw cycles; aliquot for single use |
| Batch-to-Batch Consistency | MALDI-TOF mass confirmation per lot; qPCR performance within 0.5 Ct of reference lot |
For research use only, not for clinical use.
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