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| Product Name | CHAPS Zwitterionic Detergent for Membrane Protein Work |
| Catalog No. | RERE-0027 |
| Description | A non-denaturing amphoteric surfactant built from a cholic acid core linked to a sulphobetaine group. It keeps proteins native while disrupting membrane integrity and protein-protein contacts, and its low absorbance in the ultraviolet makes it the first detergent to reach for when membrane proteins will be tracked by UV detection. The low micelle mass of 6150 and high critical micelle concentration of 6-10 mM mean the reagent is removed completely by ordinary dialysis, and 2-4% working concentrations are standard in isoelectric focusing gels. |
| Intended Use | Solubilisation of membrane proteins, disruption of protein-protein interactions, and sample preparation for isoelectric focusing and two-dimensional electrophoresis. |
| Principle / Technology | Zwitterionic detergent action: micelles envelope hydrophobic membrane regions while the charged headgroups keep the complex water-soluble and non-denaturing. |
| Sample Type | Membrane fractions, cell lysates and 2D electrophoresis sample preparations |
| Performance Range / Specifications | Critical micelle concentration 6-10 mM; micelle molecular weight 6150; water solubility up to 100 mg/mL; low ultraviolet absorbance |
| Specificity | Non-denaturing amphoteric character preserving native protein structure |
| Reaction Conditions / Protocol | Dissolve in the working buffer to the required concentration, commonly 2-4% (w/v) in isoelectric focusing gel applications, and proceed with the solubilisation or electrophoresis protocol. |
| Components / Formulation | Solid detergent reagent |
| Storage Conditions | Room temperature |
| Shelf Life | 24 months |
| Package Specifications | 1 g, 5 g, 25 g and 100 g packs |
| Product Form | White crystalline solid |
| Quality Control | Released against identity and purity criteria |
| Key Features | Non-denaturing solubilisation that preserves native structure; lowest-UV detergent choice for membrane protein tracking; fully dialysable after use; standard reagent for focusing and 2D gels |
| Purity | High-purity biochemical grade |
| Concentration | Solid; dissolve to 2-4% (w/v) for focusing gel use |
| Molecular Weight | 614.9 |
| Source / Origin | Synthetic zwitterionic cholic acid derivative |
| pH Range / Optimal pH | Neutral in solution |
| Shipping Conditions | Room temperature |
| Expiration Date / Stability | Two years at room temperature |
| Regulatory / Compliance | Research-use reagent |
| Compatibility | Compatible with isoelectric focusing, 2D electrophoresis and standard lysis buffers; removable by dialysis thanks to the high critical micelle concentration |
| Application Notes / Precautions | Prepare fresh solutions where possible; confirm compatibility with the downstream MS or electrophoresis platform; store tightly sealed against moisture uptake. |
| Batch-to-Batch Consistency | Lot-numbered packaging |
| Model Type | Zwitterionic detergent reagent |
| Starting Material / Tissue Site | Membrane fractions and protein samples needing gentle solubilisation |
| Delivery Standard | Shipped at ambient temperature |
| Readouts / Downstream Assays | Downstream gel imaging, UV protein quantification or activity assays after solubilisation |
For research use only, not for clinical use.
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