| Product Name | Caspase-3 Assay Reagent for Live Cells |
| Catalog No. | CATR-HMM-0018 |
| Description | Compared to other fluorescence-based (FLICA) analysis substrates or fluorescence inhibitors for Caspase, the substrate provided in the reagent detects Caspase-3/7 activity without inhibiting the apoptosis process in intact cells. The substrate consists of a fluorescent DNA dye coupled to the DEVD recognition sequence of Caspase-3/7. The substrate is initially non-fluorescent and enters the cytoplasm through the cell membrane. In apoptotic cells, Caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus, labels DNA, and emits bright green fluorescence. The substrate is dual-functional, capable of detecting Caspase-3/7 activity while also visualizing morphological changes in the nucleus during apoptosis. SuperView staining is compatible with formalin fixation and subsequent immunofluorescence experiments. |
| Application | This product provides an effective tool for detecting apoptosis based on Caspase-3/7 activity, suitable for fluorescence microscopy and flow cytometry analysis. |
| Spectral Parameters | Ex/Em = 500/530 nm (DNA-bound) |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Storage | Store at 4°C. |
| Shelf Life | 1 year |
| Features | Visualization of morphological changes in cell apoptosis; compatible with subsequent immunofluorescence staining experiments. |
For research use only, not for clinical use.
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