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| Product Name | Caspase 3 Activity Assay Reagent |
| Catalog No. | CCAT-HMM-0036 |
| Description | Caspase 3, also known as CPP32, Yama, or apoptin, sometimes written as caspase-3 or caspase 3, belongs to the CED-3 subfamily of the caspase family and is a key enzyme in the process of apoptosis. Caspase 3 is the most researched caspase in mammalian cells. Caspase 3 can cleave procaspase 3, 6, 7, and 9, and can directly and specifically cleave many caspase substrates, including PARP (poly(ADP-ribose) polymerase), ICAD (Inhibitor of caspase-activated deoxyribonuclease), gelsolin, and fodrin, among others. These caspase-3-mediated protein cleavages are an important component of the molecular mechanisms of apoptosis. Additionally, caspase-3 plays a key role in nuclear apoptosis, including chromatin condensation and DNA fragmentation. Caspase-3 also plays a critical role in cell blebbing. |
| Application | This product uses spectrophotometry to detect caspase 3 enzyme activity in cell or tissue lysates or purified caspase 3 enzyme activity. |
| Applicable Instruments | Enzyme-linked immunosorbent assay reader, spectrophotometer |
| Storage | Store at -20°C away from light. |
| Notes | Ac-YVAD-pNA should be avoided from repeated freeze-thaw cycles. Please note to aliquot appropriately. It is recommended to dilute the sample with water at a 1:1 ratio before determining protein concentration using the Bradford method to minimize interference from DTT on protein concentration measurement. pNA (Chinese name: 4-nitroaniline) is toxic to humans. Handle with extreme caution and ensure proper protective measures to avoid direct contact with the skin or inhalation. pNA (10 mM) may solidify and adhere to the bottom, walls, or lid of centrifuge tubes at low temperatures such as 4°C or ice bath conditions. It can be warmed in a water bath at 20–25°C for a short time until completely dissolved before use. For your safety and health, please wear a lab coat and disposable gloves during handling. |
For research use only, not for clinical use.
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