| Product Name | Apoptosis Assay Reagent |
| Catalog No. | CCAT-HMM-0006 |
| Description | Annexin V is a Ca²⁺-dependent phospholipid-binding protein with a molecular weight of 35–36 kDa that selectively binds to phosphatidylserine (PS). PS is primarily distributed on the inner side of the cell membrane, adjacent to the cytoplasm. During the early stages of apoptosis, various cell types translocate phosphatidylserine to the cell surface, exposing it to the extracellular environment. Annexin V labeled with a green fluorescent probe binds to the translocated PS, enabling the detection of phosphatidylserine translocation—a key feature of apoptosis—using flow cytometry or fluorescence microscopy. |
| Application | This product provides a rapid and convenient method for detecting apoptosis levels by labeling early apoptotic cells (green) and necrotic or late apoptotic cells (red). |
| Applicable Instruments | Flow cytometer, fluorescence microscope |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; sterile ddH₂O |
| Storage | Store at 4°C away from light. Do not freeze. |
| Features | Wide selection: Wide range of colors to choose from; Good stability: Strong fluorescence brightness and resistant to quenching; Good sorting effect: Strong specificity and obvious cell sorting. |
| Notes | 1. Before use, briefly centrifuge the product to the bottom of the tube, then proceed with subsequent experiments. 2. To reduce the rate of apoptosis, the incubation process can be performed on ice, but the incubation time should be extended to at least 30 minutes. 3. Since apoptosis is a rapid process, it is recommended that samples be analyzed within 1 hour after staining. 4. For adherent cells, digestion is a critical step. When inducing apoptosis in adherent cells, if there are floating cells, collect both floating and adherent cells and combine them for staining. Handle adherent cells carefully to avoid causing damage. If trypsin digestion time is too short, cells may require vigorous pipetting to detach, which can damage cell membranes and lead to excessive PI uptake; 5. After trypsin digestion of adherent cells, it is recommended to allow them to recover for approximately 30 minutes under optimal culture conditions and medium before staining to avoid false positives. 6. To minimize cell loss during washing, use a larger tip over a smaller tip when aspirating. 7. The optimal dye concentration is determined by specific experimental requirements. 8. All fluorescent dyes are subject to photobleaching. Please avoid exposure to light during storage and use to slow down photobleaching. 9. For your safety and health, please wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
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