| Product Name | Cell Cycle Assay Reagent (Blue Fluorescence) |
| Catalog No. | CCAT-HMM-0019 |
| Description | The cell cycle refers to the entire process that continuously dividing cells undergo from the end of one mitosis to the end of the next mitosis. During this process, the cell's genetic material is replicated and duplicated, and is evenly distributed to the two daughter cells at the end of division. The cell cycle can be further divided into interphase and mitotic phase (M phase). Interphase is commonly subdivided into the resting phase (G0), the pre-DNA synthesis phase (G1), the DNA synthesis phase (S), and the post-DNA synthesis phase (G2). DNA cycle detection can be used to assess the status of each phase of the cell cycle, i.e., the cell proliferation status. By utilizing the property that DNA within cells can bind to fluorescent dyes (such as Cycle Blue), the different DNA content in each phase of the cell cycle results in varying amounts of fluorescent dye binding, and the differing fluorescence intensities detected by flow cytometry can be used to identify cells in different phases of the cycle. During apoptosis, due to the condensation of cytoplasm and chromatin, nuclear fragmentation occurs, leading to the formation of apoptotic bodies, which alter the cell's light scattering properties. In the early stages of apoptosis, the cell's forward light scattering ability significantly decreases, while lateral light scattering ability increases or remains unchanged. In the late stages of apoptosis, both forward and lateral scattering signals decrease. Therefore, apoptosis can be observed by measuring changes in light scattering using flow cytometry. After Cycle Blue staining, assuming that the fluorescence intensity of G0/G1 phase cells is 1, the theoretical fluorescence intensity of G2/M phase cells with a double genome is 2, and the fluorescence intensity of S phase cells undergoing DNA replication is between 1 and 2. Apoptotic cells exhibit significant weak staining after Cycle Blue staining, with fluorescence intensity less than 1, due to nuclear condensation and DNA fragmentation, resulting in the loss of some genomic DNA fragments during staining. This manifests as the so-called sub-G1 peak, or apoptotic cell peak, in the fluorescence plot of flow cytometry analysis. |
| Application | This product can be used for cell cycle detection. |
| Spectral Parameters | Ex/Em (nm) = 364/454 |
| Applicable Instruments | Flow cytometer |
| Materials to Bring | Anhydrous ethanol, PBS (E-BC-R187) |
| Storage | Store at -20°C away from light. |
| Shelf Life | 1 year |
For research use only, not for clinical use.
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