| Product Name | Caspase-3 Substrate, 1 mM in DMSO |
| Catalog No. | CCAT-HMM-0015 |
| Description | The substrate consists of a fluorescent DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate is initially non-fluorescent and crosses the cell membrane into the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus, labels DNA, and emits bright green fluorescence. |
| Application | This product provides an effective tool for detecting apoptosis based on caspase-3/7 activity. |
| Spectral Parameters | Ex/Em (nm) = 500/530 (with DNA) |
| Applicable Instruments | Flow cytometer, fluorescence microscope, fluorescence enzyme-linked immunosorbent assay instrument |
| Storage | Store in a dry place away from light at -20°C. |
| Features | Good stability: High fluorescence brightness and good quenching resistance; Small batch-to-batch variation: Good control of batch-to-batch variation. |
| Notes | 1. Cells can be co-stained with Hoechst 33342 dye at a final concentration of 1 μM to produce blue fluorescence staining of the cell nucleus (excitation/emission: 346/460 nm). 2. SuperView 488 staining can be fixed with formaldehyde but is incompatible with methanol fixation. 3. SuperView 488-stained cells fixed with formaldehyde can be treated with 0.1% Triton X-100 before subsequent staining, but this may reduce the brightness of the staining. 4. For your safety and health, please wear a lab coat and disposable gloves when handling the solution. |
For research use only, not for clinical use.
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