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| Product Name | Calcium (Ca) Colorimetric Assay Kit |
| Catalog No. | ETK-YJL-0006 |
| Size | 96 T (80 samples), 48 T (32 samples) |
| Sample Type | Serum (plasma); urine; tissues |
| Detection Instrument | Microplate reader (600-620 nm, optimal detection wavelength: 610 nm) |
| Research Field | Inorganic salt ions; Plant physiology series |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-1.2 mmol/L |
| Accuracy | Inter-assay variation: 8.5% | Intra-assay variation: 4.7% |
| Sample Size | 10 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | Ca |
| Detection Time | 20 min |
Calcium (Ca) is an indispensable mineral in all living organisms and a crucial intracellular signaling molecule that governs a vast array of physiological and pathological processes. Its role extends from maintaining basic cellular functions to supporting systemic health, making the accurate quantification of calcium levels essential in both research and applied science fields.
Physiological Significance of Calcium: Approximately 99% of calcium in the human body is stored in bones and teeth, where it forms complexes like calcium phosphate and calcium carbonate to provide structural support. The remaining 1% circulates in the blood and soft tissues, and this small fraction plays a outsized role in critical biological activities. It mediates the constriction and relaxation of blood vessels, ensuring proper cardiovascular function; facilitates nerve impulse transmission, enabling communication between neurons; triggers muscle contraction, from voluntary movements to involuntary processes like heartbeat; regulates hormone and neurotransmitter secretion, maintaining endocrine balance; and supports blood clotting, preventing excessive bleeding. Beyond these, calcium signaling is the foundation of key processes such as cell adhesion, glycogen metabolism, cell proliferation and differentiation—all of which are vital for mammalian development, growth and reproduction.
Implications of Calcium Level Abnormalities: Serum calcium levels are tightly regulated within a narrow physiological range (typically 8.4-11.4 mg/dl in humans). Any deviation from this range can have serious health consequences. Low calcium levels (hypocalcemia) may arise from chronic kidney failure, vitamin D deficiency, or severe alcoholism-induced low blood magnesium levels, leading to symptoms like muscle cramps, numbness, and even cardiac arrhythmias. High calcium levels (hypercalcemia) can result from conditions such as hyperparathyroidism or bone disorders, potentially causing kidney damage, fatigue, and confusion. For researchers, understanding these abnormalities requires reliable tools to measure calcium concentrations in various biological samples.
Need for Colorimetric Assay Kits: Traditional methods for calcium detection often involve complex equipment, time-consuming protocols, or limited sensitivity. Colorimetric assay kits address these challenges by leveraging specific chemical reactions between calcium ions and chromogenic reagents—reactions that produce colored complexes with absorbance properties proportional to calcium concentration. This approach enables quantitative, rapid, and cost-effective calcium measurement, making it accessible for labs of all sizes. Our Calcium (Ca) Colorimetric Assay Kit builds on this technology, offering a optimized solution for researchers studying inorganic salt ions, plant physiology, metabolism, and disease-related calcium dysregulation.
Broad Sample Compatibility: Designed to work with multiple common biological sample types, including serum, plasma, urine, and tissues. This flexibility eliminates the need for separate kits for different sample sources, simplifying experimental workflows for researchers studying diverse biological systems.
Precise Detection Performance: Boasts a sensitivity of 0.07 mmol/L and a detection range of 0.07-1.2 mmol/L—parameters that align with the physiological concentration range of calcium in many biological samples. This ensures accurate measurement of both low and moderate calcium levels, avoiding the limitations of kits with narrow ranges or low sensitivity.
Reliable Reproducibility: Maintains strict quality control, with inter-assay variation of 8.5% and intra-assay variation of 4.7%. These low variation rates guarantee consistent results across different experiments, batches, and operators, reducing experimental errors and enhancing data credibility.
Fast Assay Workflow: Requires only 20 minutes of total detection time, from sample preparation to result readout. This rapid turnaround is ideal for high-throughput experiments or labs with tight project timelines, minimizing waiting time without compromising data quality.
Minimal Sample Requirement: Needs just 10 μL of sample per test. This feature is particularly valuable for precious or limited-volume samples (e.g., rare tissue samples, small animal plasma) where conserving sample quantity is critical for subsequent experiments.
Convenient Storage Conditions: Stable for 6 months when stored at 2-8°C and protected from light. No need for labor-intensive storage (e.g., -20°C freezers or dry ice), reducing lab storage costs and simplifying inventory management.
User-Friendly Protocol: Requires minimal hands-on time and no specialized technical expertise. The kit includes clear, step-by-step instructions, making it suitable for both experienced researchers and lab technicians new to calcium assay procedures.
Cost-Effective Sample Testing: Available in two sizes (96 T for 80 samples, 48 T for 32 samples), allowing labs to choose the format that matches their sample volume needs. This prevents overordering and reduces waste, lowering per-sample testing costs compared to one-size-fits-all kits.
Optimal Detection Wavelength: Compatible with standard microplate readers, with an optimal detection wavelength of 610 nm (and a range of 600-620 nm). Most labs already have access to microplate readers capable of measuring this wavelength, eliminating the need for additional, expensive equipment purchases.
Targeted for Key Research Fields: Specifically tailored for studies in inorganic salt ions and plant physiology—two high-demand research areas. Unlike general-purpose assay kits, this kit’s performance parameters (e.g., sensitivity, sample compatibility) are optimized for the unique needs of these fields, ensuring more relevant and reliable data.
Consistent Quality Assurance: Each kit undergoes rigorous pre-shipment testing to verify sensitivity, precision, and stability. This quality assurance process ensures that every kit meets the same high standards, giving researchers confidence in the reliability of their results.
Reduced Experimental Complexity: The colorimetric detection method eliminates the need for radioactive labels, fluorescent probes, or complex sample pretreatment (e.g., extensive purification). This not only simplifies the assay process but also reduces safety risks (e.g., radiation exposure) and associated regulatory compliance requirements.
For research use only, not for clinical use.
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