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Calcein/PI Cell Viability and Cytotoxicity Assay Kit

Cat.No: REKI-0037 Datasheet

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Product Details Related Products
Product Name Calcein/PI Cell Viability and Cytotoxicity Assay Kit
Catalog No. REKI-0037
Description Two-color kit combining esterase-reactive Calcein with propidium iodide, so living and membrane-damaged cells are counted in the same well. Viable cells with intact membranes convert the calcein reagent into green fluorescence, whereas cells with compromised membranes admit PI and fluoresce red, giving paired viability and cytotoxicity readouts across 100-test, 500-test and 2500-test formats. Store at -20 °C; shelf life is 12 months.
Intended Use Paired measurement of live cell activity and cytotoxicity in the same sample
Principle / Technology Intracellular esterases generate green calcein signal in living cells while PI enters membrane-compromised cells to emit red fluorescence
Specificity 100 T / 500 T / 2500 T; green and red fluorescence channels; 12 month shelf life
Reaction Conditions / Protocol Add the combined staining solution to the culture, incubate protected from light and record both channels
Storage Conditions -20 °C
Package Specifications 100 T / 500 T / 2500 T
Key Features Two-color live and dead discrimination; large-volume screening formats; 12 month shelf life

For research use only, not for clinical use.

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