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Calcein-AM/PI Cell Viability and Cytotoxicity Assay Kit

Cat.No: REKI-0038 Datasheet

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Product Details Related Products
Product Name Calcein-AM/PI Cell Viability and Cytotoxicity Assay Kit
Catalog No. REKI-0038
Description Dual-fluorescence viability kit in which Calcein-AM labels living cells green through intracellular esterase conversion while PI stains membrane-damaged cells red. The paired signals allow both the viable fraction and the dead fraction of each well to be quantified in a single measurement, which is convenient for cytotoxicity titrations. Offered in 500-test and 1000-test formats; store at -20 °C for up to 12 months.
Intended Use Combined viability and cytotoxicity readout in titration and screening assays
Principle / Technology Calcein-AM reports esterase-active intact cells in green and PI reports membrane-compromised cells in red
Specificity 500 T / 1000 T; green and red fluorescence channels; 12 month shelf life
Reaction Conditions / Protocol Apply the staining cocktail to the cell culture, incubate protected from light and image or read both fluorescence channels
Storage Conditions -20 °C
Package Specifications 500 T / 1000 T
Key Features Single-stain-step live and dead scoring; screening-scale pack sizes; 12 month shelf life

For research use only, not for clinical use.

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