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| Product Name | AO/PI Double Staining Kit |
| Catalog No. | RESK-0162 |
| Description | The AO/PI Double Staining Kit is a fluorescence staining-based cell viability detection tool whose core components are acridine orange (AO) staining solution and propidium iodide (PI) staining solution mixed in an optimized ratio. Through dual staining, the kit rapidly and accurately distinguishes normal cells, apoptotic cells and necrotic cells, and it is widely used in life science research scenarios such as drug screening, cell culture monitoring and environmental stress analysis. Spectral properties: AO Ex/Em 500/526 nm (bound to DNA); AO Ex/Em 460/650 nm (bound to RNA); PI Ex/Em 493/636 nm (without DNA); PI Ex/Em 535/617 nm (bound to DNA). |
| Intended Use | Rapid fluorescence-based discrimination of normal, apoptotic and necrotic cells for cell viability analysis in drug screening, cell culture monitoring and environmental stress analysis. |
| Principle / Technology | Dual fluorescence staining with acridine orange (AO) and propidium iodide (PI) in an optimized ratio. Cooperative dual staining combined with a fluorescence resonance energy transfer (FRET) mechanism achieves clear separation of the green (AO) and red (PI) fluorescence signals, enabling accurate identification and quantification of normal, apoptotic and necrotic cells. |
| Detection Method | Fluorescence staining; compatible with fluorescence-based automated cell counters for automatic recognition and quantitative analysis. |
| Sample Type | Cultured cells. |
| Performance Range / Specifications | Safe and efficient: PI replaces the ethidium bromide (EB) used in traditional AO/EB kits, avoiding the high carcinogenicity and spectral interference of EB and greatly reducing experimental risk. Accurate quantification: cooperative dual staining combined with a FRET mechanism achieves clear separation of green (AO) and red (PI) fluorescence, is compatible with automatic recognition and quantitative analysis by mainstream cell counters, and provides data reliability far exceeding the traditional trypan blue single-staining method. Convenient operation: ready-to-use dual-staining reagent with no complex sample pretreatment, avoiding dilution errors; compatible with parameter adjustment of fluorescence cell counters (e.g., exposure time and gain) to flexibly meet different experimental needs. |
| Reaction Conditions / Protocol | Stain cells directly with the ready-to-use AO/PI dual-staining reagent; no complex sample pretreatment is required. Instrument parameters such as exposure time and gain can be adjusted on the fluorescence cell counter as needed. |
| Components / Formulation | Acridine orange (AO) staining solution and propidium iodide (PI) staining solution mixed in an optimized ratio. |
| Storage Conditions | Store at 2-8 °C. |
| Shelf Life | 2 years |
| Package Specifications | 100 T 5 x 100 T |
| Product Form | Liquid, ready-to-use dual-staining reagent. |
| Expiration Date / Stability | Valid for 2 years when stored at 2-8 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with mainstream fluorescence cell counters (with adjustable exposure time and gain). |
| Application Notes / Precautions | Fluorescence characteristics: AO Ex/Em 500/526 nm (with DNA) and 460/650 nm (with RNA); PI Ex/Em 493/636 nm (without DNA) and 535/617 nm (with DNA). |
For research use only, not for clinical use.
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