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| Product Name | CA Buffer, pH 7.5 |
| Catalog No. | RERE-0425 |
| Description | Caspases are cysteine proteases whose active sites are destroyed by oxidation, which is why this dedicated analysis buffer folds DTT into a sucrose-HEPES-salt matrix. Sucrose cushions organelles still suspended in the lysate, HEPES holds pH 7.5, and the chloride salts of potassium and magnesium tune ionic conditions for enzyme-substrate engagement. The result is a medium in which caspase activity survives from lysis through measurement. |
| Intended Use | Buffering of caspase activity assays and related cysteine protease measurements. |
| Principle / Technology | DTT maintains the reduced catalytic cysteine while HEPES-sucrose-salt chemistry preserves cellular context. |
| Specificity | Composed of sucrose, HEPES, potassium chloride, magnesium chloride and DTT; pH 7.5. |
| Reaction Conditions / Protocol | Use as the assay and lysis buffer in caspase analysis protocols. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Reducing environment protects caspase active sites; five-component physiological matrix; pH 7.5. |
For research use only, not for clinical use.
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