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Broad-Range dsDNA Fluorescence Quantification Kit

Cat.No: NG-QCY-0045 Datasheet

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Product Details Related Products
Product Name Broad-Range dsDNA Fluorescence Quantification Kit
Catalog No. NG-QCY-0045
Description A fluorescence assay for broad-range quantification of double-stranded DNA. The dye preferentially binds dsDNA and tolerates common contaminants including salts, free nucleotides, proteins, solvents and detergents.
Intended Use broad-range quantification of double-stranded DNA
Principle / Technology Fluorescent dye binding to double-stranded DNA
Detection Method Fluorometric measurement
Sample Type Double-stranded DNA samples
Performance Range / Specifications Total dsDNA range: 4-2,000 ng; sample concentration range: 0.2-2,000 ng/μL
Sensitivity / LOD Quantifies as little as 4 ng total dsDNA within the stated linear range
Specificity Preferential response to dsDNA over RNA; tolerant of common sample contaminants.
Reaction Conditions / Protocol Dilute the fluorescence reagent with assay buffer, add the dsDNA sample, incubate at room temperature and measure fluorescence.
Components / Formulation Fluorescence detection reagent, assay buffer and dsDNA standards
Storage Conditions Store at 2-8°C protected from light; store the higher-concentration standard at −30 to −15°C for long-term storage.
Package Specifications 100 assays; 500 assays
Product Form Multi-component fluorescence quantification kit
Quality Control Standards and detection reagents are functionally tested for quantitative performance.
Key Features Broad dynamic range; strong dsDNA selectivity; room-temperature workflow; dye binding reaches saturation within approximately 2 minutes; contaminant tolerance.
Shipping Conditions Ship with cold packs.
Compatibility Qubit fluorometers and compatible fluorescence readers.
Batch-to-Batch Consistency Quantitative performance and standard response are controlled across production batches.

For research use only, not for clinical use.

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