| Product Name | Biotin TUNEL Apoptosis Reagent |
| Catalog No. | CCAT-HMM-0013 |
| Description | When cells undergo apoptosis, certain DNA endonucleases are activated. These endonucleases cleave genomic DNA between nucleosomes, producing DNA fragments of 180 bp to 200 bp. The broken DNA fragments expose a large number of sticky 3'-OH ends, which can bind to biotin (Biotin)-dUTP, which then binds to horseradish peroxidase (HRP)-labeled streptavidin (Streptavidin-HRP). Finally, under the catalysis of HRP, DAB staining is used to visualize apoptotic cells, enabling direct detection of apoptotic cells via optical microscopy. This method is referred to as Terminal-deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). |
| Application | This product can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspension cells. It selectively detects apoptotic cells, rather than necrotic cells or cells with DNA strand breaks caused by irradiation or drug treatment. |
| Applicable Instruments | Optical microscope |
| Materials to Bring | PBS buffer (1×, pH 7.4); 0.4% Triton X-100 (PBS-based); 1% Triton X-100 (PBS-prepared); Paraffin section processing reagents; 4% paraformaldehyde (PBS-prepared); Immunohistochemistry pen; 3% H₂O₂ (methanol: ddH₂O: H₂O₂ = 8:1:1; freshly prepared); Neutral resin; ddH₂O |
| Storage | Store at -20°C away from light. |
| Notes | 1. Before use, centrifuge the product briefly to the bottom of the tube, then proceed with subsequent experiments. 2. Sodium azide inhibits HRP. Do not use reagents containing sodium azide in the experiment. 3. For your safety and health, please wear a lab coat and disposable gloves when operating. |
For research use only, not for clinical use.
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