Human CD4+ T Cell Isolation Kit ( Negative Selection )

CD4+ T cells, a critical subset of T lymphocytes, serve as central regulators of adaptive immune responses—playing pivotal roles in coordinating antibody production by B cells, activating cytotoxic CD8+ T cells, and modulating the function of macrophages and dendritic cells. Their dysregulation is closely linked to the pathogenesis of numerous diseases, including autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis), infectious diseases (e.g., HIV, which specifically targets and depletes CD4+ T cells), and cancers (where impaired CD4+ T cell-mediated immune surveillance enables tumor progression). As such, the ability to isolate highly pure, viable, and functionally intact CD4+ T cells is essential for advancing research in immunology, translational medicine, and drug discovery.

Traditional methods for CD4+ T cell isolation, such as density gradient centrifugation alone or column-based positive selection, have significant limitations:
Density gradient centrifugation (e.g., using Ficoll-Paque) can only enrich peripheral blood mononuclear cells (PBMCs) but fails to specifically purify CD4+ T cells, resulting in contamination by CD8+ T cells, B cells, and monocytes.
Column-based positive selection, which labels CD4+ T cells directly with magnetic beads, often leaves residual beads or antibodies on the cell surface. These contaminants can interfere with downstream applications like flow cytometry (by altering antigen detection), cell culture (by inducing unintended activation), or functional assays (e.g., cytokine secretion, T cell proliferation).

Against this backdrop, negative selection technology has emerged as a superior approach for CD4+ T cell isolation. Unlike positive selection, negative selection targets and removes unwanted cells (rather than labeling the desired CD4+ T cells), ensuring the isolated CD4+ T cells remain "untouched"—free of bead or antibody binding. This preserves their native phenotype and functional integrity, making them ideal for sensitive downstream experiments.

Our Human CD4+ T Cell Isolation Kit (Negative Selection) leverages this advanced immunomagnetic negative selection principle. It is designed to address the unmet needs of researchers seeking consistent, high-quality CD4+ T cell preparations from human biological samples, supporting a wide range of applications from basic immune function studies to preclinical drug testing.