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| Product Name | His-tag Protein Purification Beads (IDA-Co) |
| Catalog No. | SM-HMM-0059 |
| Description | Cobalt Ion Chelated Magnetic Beads for His-tag Protein Purification |
| Features | Direct purification of target proteins from crude samples, greatly reducing the purification time; Easily control the concentration and volume of target protein; High purity of target protein can be obtained in one step; High stability of parallel operation, which is convenient for high throughput and large-scale protein purification; High yield of target protein can be obtained; Reusable and easy to regenerate. |
| Storage | 2-8°C |
| Shelf Life | 2 years |
| Average Particle Size | 30-150 µm |
| Concentration | 10% (v/v) |
| Product Introduction | These magnetic beads are charged with cobalt ions (Co2+) via an immodiacetic acid (IDA) linker, creating a high-affinity matrix for the purification of recombinant proteins containing a polyhistidine (His) tag (typically 6xHis). The Co2+ ions offer superior specificity over traditional nickel-charged beads, reducing non-specific binding of host cell proteins, especially those containing endogenous metal-binding motifs. The magnetic format enables rapid, batch-mode purification under native or denaturing conditions directly from crude lysates without the need for columns or centrifugation. |
| Product Application | • One-step purification of His-tagged proteins from bacterial, mammalian, or insect cell lysates. • Small-scale screening of expression conditions and solubility. • Pull-down assays to capture His-tagged proteins and their interactors. • Automated, high-throughput protein purification workflows. |
| Product Advantages | • Enhanced Specificity (Cobalt): Cobalt chemistry provides higher selectivity for the His-tag compared to nickel, resulting in purer protein preparations with fewer contaminating proteins. • High Binding Capacity: The beads offer a high density of charged metal ions, enabling efficient capture even from dilute lysates or for high-yield expressions. • Magnetic Workflow Efficiency: Enables rapid washing and elution steps using a simple magnet, streamlining the process and reducing hands-on time significantly. • Gentle Elution Options: Proteins can be eluted under native conditions using low concentrations of imidazole or competitively with EDTA, helping to preserve protein structure and activity. |
For research use only, not for clinical use.
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