- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Mouse CD8+ T Cell Isolation Kit ( Negative Selection ) |
| Catalog No. | SM-HMM-0043 |
| Description | The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field. Our Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions. |
| Features | Simple and fast: the target cells can be sorted in 15 minutes; No need for a separation column: rapid separation of destination cells can be achieved by using a magnetic separator; High purity: the purity of sorted cells can be more than 95%; High activity: more than 99% active cells can be obtained after sorting, and the cell function is good. |
| Storage | Store at 2-8°C, do not freeze. |
| Applications | This kit is suitable for sorting mouse spleen and lymph node CD8+ T cells. |
| Product Introduction | This kit utilizes a sophisticated negative selection strategy to rapidly isolate untouched CD8+ T cells from mouse spleen or lymph node suspensions. By depleting non-target cells (including CD4+ T cells, B cells, NK cells, monocytes, granulocytes, and erythrocytes) using a cocktail of biotinylated antibodies and anti-biotin magnetic beads, it yields highly pure, functionally unaltered CD8+ T cells. These cells are ideal for downstream applications where receptor engagement and activation status must remain pristine, such as in vitro stimulation, cytokine profiling, and adoptive transfer experiments. |
| Product Application | • Isolation of untouched CD8+ T cells for functional assays (proliferation, cytotoxicity) • Preparation of cells for adoptive transfer models • Gene expression studies on purified, unstimulated populations • Flow cytometry analysis with no antibody interference |
| Product Advantages | • Purity & Viability: Delivers high-purity (>90%) and high-viability CD8+ T cell populations without antibody binding to the target cells, preserving their native state. • Rapid Magnetic Workflow: Employs a fast, column-free magnetic separation process that can be completed in under 30 minutes, saving valuable time. • Untouched & Functional: Cells are not labeled with antibodies, leaving all surface receptors free for subsequent experimental manipulation, ensuring more physiologically relevant results. • Scalable & Flexible: The protocol is easily scalable from small (10^7) to large (10^9) total cell numbers, accommodating a wide range of experimental needs. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagenoTech. All rights reserved.
