Strep-tag II Protein Purification Beads

Cat.No: SM-HMM-0063 Datasheet

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Product Name	Strep-tag II Protein Purification Beads
Catalog No.	SM-HMM-0063
Description	This product is a new functional material designed for efficient and rapid purification of Strep-tag II protein. Strep-tag II consists of only eight chemically balanced amino acid short peptides (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), making the effect on the structure and activity of the recombinant protein negligible. Therefore, it is not necessary to remove Strep-tag II in the target protein during the purification process. At the same time, the target protein can be purified under physiological conditions. Compared with other tags, these mild purification parameters can preserve the biological activity of the protein and obtain the target protein with a purity of more than 99%.
Storage	2- 30°C (for long term storage, 2℃-8°Cis recommended)
Average Particle Size	30-150 µm
Preservation Fluid	1 x PBS(0.I% Tween-20+0.05% NaN3)
Concentration	10% (v/v)
Applications	Can be used for the isolation and purification of Strep-Tag II tagged proteins from any expression system, including baculoviruses, mammalian cells, yeast and bacteria.

Product Introduction	These beads are conjugated with a high-affinity engineered streptavidin derivative that specifically binds the 8-amino acid Strep-tag II peptide. This system allows for the gentle, one-step purification of recombinant proteins fused with Strep-tag II directly from crude cell lysates under native conditions. Elution is achieved with a mild, non-denaturing biotin analogue (desthiobiotin).
Product Application	• One-step purification of Strep-tag II fusion proteins • Pull-down assays for protein-protein interaction studies • Immobilization of proteins for enzymatic or binding assays • Sample preparation for structural biology or mass spectrometry
Product Advantages	• Exceptional Specificity & Purity: The engineered streptavidin-Strep-tag II interaction is highly specific, resulting in extremely pure protein preparations with minimal non-specific binding. • Gentle Elution Conditions: Proteins are eluted under native, non-denaturing conditions using desthiobiotin, preserving protein structure, activity, and complexes. • Magnetic Workflow Efficiency: The magnetic format enables rapid batch purification without the need for columns, centrifugation, or filtration, streamlining the process. • Reusable Beads: The binding is reversible, and the beads can be regenerated and reused multiple times after elution, offering excellent cost-effectiveness for routine purifications.

The Strep-tag system is a powerful tool for recombinant protein work, and Alta DiagnoTech offers integrated solutions. Our Strep-Tag II protein purification beads are designed for high-purity isolations. To ensure optimal protein expression and solubility for purification, we provide a variety of expression vectors containing the Strep-tag II sequence and compatible E. coli expression strains. For efficient cell lysis prior to purification, our specialized bacterial protein extraction reagents are highly recommended. Finally, to analyze and verify your purified protein, our BCA protein assay kit and SDS-PAGE reagents complete the workflow.

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For research use only, not for clinical use.