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| Product Name | Bacterial Membrane Vesicle Isolation Kit (for Culture Medium Supernatant) |
| Catalog No. | RESK-0618 |
| Description | Most bacteria release membrane vesicles (MVs) of approximately 200-400 nm in diameter. These vesicles carry specific functional molecules that transport virulence factors, participate in horizontal gene transfer, and deliver host defense factors, playing an important role in bacteria-host communication. Designed around the structural features of bacterial MVs, this kit is suitable for the diverse vesicles produced by Gram-negative and Gram-positive bacteria and enables rapid isolation of bacteria-derived MVs from culture medium supernatant. |
| Intended Use | Rapid isolation of bacterial membrane vesicles (MVs) from bacterial culture medium supernatant for routine laboratory vesicle research. Isolated MVs are suitable for particle size analysis, nucleic acid extraction, and Western Blot. |
| Principle / Technology | Affinity resin chromatography that captures a broad range of membrane vesicles from bacterial culture medium, delivering high yield and high purity. |
| Detection Method | Downstream analysis: particle size analysis (NTA), transmission electron microscopy (TEM), nucleic acid extraction, Western Blot. |
| Sample Type | Bacterial culture medium supernatant (Gram-negative and Gram-positive bacteria); 10 mL of bacterial culture per reaction. |
| Performance Range / Specifications | One reaction (1 T) processes 10 mL of bacterial culture and typically recovers approximately 5-30 billion total vesicle particles. TEM analysis shows typical cup-shaped (saucer-like) vesicle morphology with a diameter distribution of 50-150 nm (demonstrated for E. coli- and S. aureus-derived vesicles). |
| Specificity | Suitable for the different vesicle types produced by Gram-negative and Gram-positive bacteria. |
| Storage Conditions | 2-8 °C. |
| Shelf Life | 12 months at 2-8 °C. |
| Package Specifications | 10 T, 20 T |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Isolated MVs can be used directly for particle size analysis, nucleic acid extraction, and Western Blot. |
For research use only, not for clinical use.
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