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| Product Name | Automation-Ready Plate-Format Total RNA Library Prep Kit (dNTP-based) |
| Catalog No. | REKI-0075 |
| Description | A premixed library preparation kit for total RNA transcriptome sequencing that runs in conventional tubes or sealed 96-well plates. Because every reagent arrives pre-formulated, the workflow suits automated liquid handlers, enabling around one thousand prepared libraries per day when paired with a pipetting workstation. The kit contains RNA fragmentation, reverse transcription, double-stranded cDNA synthesis and library amplification reagents, and links directly upstream to mRNA enrichment or rRNA depletion modules. All components pass functional QC to keep library yield and insert-size distribution stable across lots. |
| Intended Use | Total RNA transcriptome library construction; high-throughput automated library preparation; upstream connection to mRNA purification or rRNA depletion workflows. |
| Principle / Technology | Fragmented RNA is reverse transcribed and converted into double-stranded cDNA, adapter ligated and PCR enriched to yield sequencing-ready total RNA libraries. |
| Sample Type | Total RNA after mRNA enrichment or rRNA depletion |
| Performance Range / Specifications | Daily output of roughly 1000 libraries with an automated workstation; stable amplification across lots. |
| Specificity | dNTP-based second-strand chemistry; tube and plate formats; no light protection required. |
| Reaction Conditions / Protocol | Fragment RNA, reverse transcribe, synthesise double-stranded cDNA, ligate adapters and amplify libraries, then bead-purify. |
| Components / Formulation | RNA fragmentation reagent, reverse transcription reagent, double-stranded cDNA synthesis reagent, library amplification reagent. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T, 96 T (plate), 96 T (automation) |
| Product Form | Premixed reagents or sealed reaction plate |
| Key Features | Peel-and-load plates; no manual formulation; dual sequencing-platform compatibility; QC-verified reproducibility. |
| Compatibility | Compatible with mainstream short-read sequencing platforms and automated pipetting workstations |
| Readouts / Downstream Assays | Total RNA-seq libraries for transcriptome profiling |
For research use only, not for clinical use.
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