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| Product Name | Automation-Ready Plate-Format Strand-Specific RNA Library Prep Kit (dUTP-based) |
| Catalog No. | REKI-0074 |
| Description | A premixed, plate-format library preparation kit that builds strand-specific RNA sequencing libraries on both major short-read sequencing platforms. The kit ships in sealed reaction plates or tube premixes, so no on-bench formulation or aliquoting is required; laboratories simply peel the seal and load. Fragmentation, reverse transcription, dUTP-based directional second-strand synthesis and library amplification reagents are all included, and the workflow connects directly to mRNA enrichment or rRNA depletion modules. The reverse transcription module delivers high strand specificity without actinomycin D, improving operator safety while preserving directional information across the transcriptome. |
| Intended Use | Strand-specific transcriptome sequencing library construction; mRNA or rRNA-depleted RNA sequencing; automated high-throughput library preparation on liquid-handling workstations. |
| Principle / Technology | RNA is fragmented, reverse transcribed, and converted to directional double-stranded cDNA through dUTP incorporation that blocks the second strand during amplification, preserving strand-of-origin information; adapter-ligated libraries are then enriched by PCR. |
| Sample Type | Total RNA following mRNA enrichment or rRNA depletion |
| Performance Range / Specifications | High strand specificity without actinomycin D; stable library yields and reproducible insert-size distributions across plates. |
| Specificity | dUTP-based directional chemistry; compatible with tube premix and sealed 96-well plate formats. |
| Reaction Conditions / Protocol | Fragment RNA, reverse transcribe, synthesise directional second strand, amplify adapters-ligated libraries, then purify with dedicated beads. |
| Components / Formulation | RNA fragmentation reagent, reverse transcription reagent, directional second-strand synthesis reagent, library amplification reagent. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T, 96 T (plate), 96 T (automation) |
| Product Form | Premixed reagents or sealed reaction plate |
| Key Features | Sealed plates suit automation workstations; no actinomycin D required; connects to mRNA enrichment or rRNA depletion modules; consistent lot-to-lot performance. |
| Compatibility | Compatible with mainstream short-read sequencing platforms; adapts to automated pipetting workstations |
| Readouts / Downstream Assays | Strand-specific RNA-seq libraries for transcriptome quantification |
For research use only, not for clinical use.
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