Arthropod, Insect and Parasite rRNA Depletion Kit

Name: Arthropod, Insect and Parasite rRNA Depletion Kit
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Cat.No: NG-QCY-0020 Datasheet

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Product Details Related Products

Product Name	Arthropod, Insect and Parasite rRNA Depletion Kit
Catalog No.	NG-QCY-0020
Description	An RNase H-based depletion kit for removing major ribosomal RNA species from arthropod, insect and parasite total RNA while retaining coding and noncoding transcripts.
Intended Use	mRNA and noncoding RNA analysis from arthropods, insects and parasites.
Principle / Technology	Target-specific probes hybridize to rRNA, followed by RNase H digestion and DNase I treatment to remove probe-bound rRNA while preserving other RNA species.
Detection Method	Downstream RNA-seq library preparation and sequencing.
Sample Type	Intact or partially degraded total RNA from arthropods, insects and parasites.
Performance Range / Specifications	Input range 0.01–1 µg total RNA; approximately 1-hour workflow; 6, 12 or 24 reactions.
Sensitivity / LOD	Reported residual rRNA below 5%.
Specificity	Targets 5S, 5.8S, 18S, 28S, 12S and 16S rRNA while retaining mRNA and noncoding RNA.
Reaction Conditions / Protocol	Hybridize total RNA with depletion probes, digest with RNase H and DNase I, and purify the depleted RNA for library construction.
Components / Formulation	Target-specific rRNA depletion probes, RNase H digestion reagents and DNase I treatment reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	6 reactions; 12 reactions; 24 reactions.
Product Form	Multi-component rRNA depletion kit.
Key Features	Broad arthropod, insect and parasite coverage; below 5% residual rRNA; approximately 1-hour workflow; compatible with partially degraded RNA.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Suitable for coding and noncoding RNA sequencing workflows.
Application Notes / Precautions	Use purified depleted RNA promptly or store under validated low-temperature conditions to minimize degradation.

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For research use only, not for clinical use.