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| Product Name | Aprotinin, Bovine Lung, Ultra-pure |
| Catalog No. | RERE-0030 |
| Description | Aprotinin, also known as bovine pancreatic trypsin inhibitor, is a competitive, reversible serine protease inhibitor that forms a stable complex with target enzymes and blocks the active site; most aprotinin-protease complexes dissociate only under extreme pH conditions. The 58-residue polypeptide contains three disulfide bonds and is supplied as an ultra-pure powder isolated from bovine lung. An animal-component-free recombinant version expressed in E. coli is also available from the same catalogue family. |
| Intended Use | Protection of proteins during extraction and purification; stabilisation of protein preparations against serine protease activity |
| Principle / Technology | Competitive, reversible binding that masks the active site of serine proteases |
| Detection Method | Trypsin, chymotrypsin, plasmin, kallikrein and related serine proteases |
| Sample Type | Cell and tissue lysates; protein preparations during purification |
| Performance Range / Specifications | Activity greater than 4500 KIU per mg, consistent with pharmacopoeial aprotinin standards |
| Specificity | Narrow-spectrum inhibitor directed at serine proteases; does not affect metalloproteases or aspartyl proteases |
| Reaction Conditions / Protocol | Dissolve in water at up to 10 mg per mL; add to extraction buffers at the concentration suited to the target application |
| Components / Formulation | Lyophilised aprotinin powder, 5 mg or 50 mg per vial |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 24 months |
| Package Specifications | 5 mg; 50 mg |
| Product Form | Greyish-white to white powder |
| Quality Control | Activity assayed in KIU per mg; identity and purity confirmed on the lot record |
| Key Features | Native protein isolated from bovine lung; high specific activity; reversible inhibition allowing downstream removal by dialysis |
| Purity | Ultra-pure grade |
| Concentration | Dissolves in water at 10 mg per mL |
| Activity / Unit Definition | Greater than 4500 KIU per mg |
| Molecular Weight | 6511.51 |
| Source / Origin | Native protein purified from bovine lung |
| Shipping Conditions | Ambient temperature or chilled packs as required by destination |
| Expiration Date / Stability | 24 months at 2 to 8°C as supplied |
| Regulatory / Compliance | For research use only |
| Compatibility | Compatible with protein extraction, purification and analytical workflows; removable by dialysis |
| Application Notes / Precautions | Animal-derived material; handle using standard biosafety practices for bovine-origin proteins |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Bovine lung tissue |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction and purification with preserved analyte integrity |
For research use only, not for clinical use.
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