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| Product Name | Apoptosis and Necrosis Detection Reagent |
| Catalog No. | CCAT-HMM-0022 |
| Description | During apoptosis, chromatin condenses. Hoechst 33342 can penetrate the cell membrane, and after staining, the fluorescence of apoptotic cells is significantly stronger than that of normal cells. Propidium Iodide (PI) cannot penetrate the cell membrane and cannot stain normal cells or apoptotic cells with intact cell membranes. However, for necrotic cells, whose cell membrane integrity is lost, Propidium Iodide (PI) can stain necrotic cells. After double staining with these two dyes, when detected using a flow cytometer or fluorescence microscope, normal cells exhibit weak red fluorescence + weak blue fluorescence, apoptotic cells exhibit weak red fluorescence + strong blue fluorescence, and necrotic cells exhibit strong red fluorescence + strong blue fluorescence. |
| Application | This product provides a rapid and convenient method for detecting both apoptosis and necrosis. |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Storage | Store at 4°C away from light. |
| Shelf Life | 6 months |
| Features | Staining is quick and convenient, with both dyes requiring only 20-30 minutes for staining, which can be completed in a single step. Easy to use, no dilution or other preparation processes are required when using a flow cytometer for detection, and no other solutions need to be prepared. |
| Notes | Testing should be performed as soon as possible after staining. For your safety and health, please wear a lab coat and disposable gloves when performing this procedure. |
For research use only, not for clinical use.
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