| Product Name | AO/PI Cell Viability Assay Reagent |
| Catalog No. | CATR-HMM-0030 |
| Description | AO is a classic, highly sensitive, and specific fluorescent dye that can label DNA and RNA. This dye has membrane permeability, enabling it to penetrate cell membranes and stain DNA and RNA. Therefore, AO is commonly used for fluorescent staining detection of DNA and RNA within cells. AO binds to nucleic acids in two primary ways: insertion binding and electrostatic attraction. Insertion binding refers to AO inserting itself between the base pairs of DNA double strands, with a fluorescence emission peak at 530 nm, emitting green fluorescence upon excitation. Electrostatic attraction refers to the electrostatic attraction between positively charged AO and the negatively charged phosphate groups of RNA or single-stranded DNA, with a fluorescence emission peak at 640 nm, emitting red fluorescence upon excitation. A small amount of binding may result in orange-yellow or orange-red fluorescence. In this reagent, AO binds to nucleic acids via insertion binding, thus emitting green fluorescence upon excitation. After AO staining, the nuclei of normal cells exhibit uniform green or yellow-green fluorescence. In apoptotic cells, due to chromatin condensation or fragmentation into irregularly sized fragments forming apoptotic bodies, AO stains them with dense, intensely stained yellow-green fluorescence or yellow-green fragment particles. In necrotic cells, yellow fluorescence is weakened or even absent. PI is a non-cell membrane-permeable fluorescent dye with a maximum excitation wavelength of 535 nm after binding to DNA, with a maximum emission wavelength of 617 nm. It cannot penetrate the cell membrane of biologically active cells but can reach the nucleus of necrotic cells due to the lack of membrane integrity, embedding into the DNA double helix of necrotic cells to form PI-DNA complexes and produce red fluorescence. Therefore, PI can only stain necrotic cells and is used to distinguish between normal cells and necrotic cells. It is important to note that apoptotic cells cannot be stained by PI due to the integrity of their cell membranes. PI staining only occurs in apoptotic cells during the late stages of apoptosis when secondary necrosis occurs. |
| Application | This product is formulated by optimizing the ratio of acridine orange (AO) staining solution and propidium iodide (PI) staining solution, based on DNA probe dual staining of cell nuclei, enabling rapid and convenient detection of cell viability. |
| Applicable Instruments | Fluorescence microscope |
| Storage | Store at 4°C away from light. |
| Shelf Life | 1 year |
| Features | This kit is easy to use and provides fast detection results. It offers ready-to-use AO/PI cell activity detection reagents. Simply mix an appropriate amount with the cell sample. There is no need for the cumbersome process of preparing and diluting various solutions, eliminating potential errors during preparation and dilution and improving the accuracy of the detection. |
| Notes | All fluorescent dyes are subject to quenching. Please avoid exposure to light to slow down fluorescence quenching. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
