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| Product Name | Anti-Biotin Nanobeads |
| Catalog No. | RESK-0455 |
| Description | Anti-Biotin Nanobeads enable positive or negative selection of cells using biotinylated antibodies. For positive selection, cells are first incubated with a biotinylated antibody so that the antibody binds the target cells, and the cells are then magnetically labeled with Anti-Biotin Nanobeads. The cell-bead suspension is loaded onto an MS column or LS column, or another suitable separation column, placed in the separation magnet of a mainstream magnetic separation system; the magnetically labeled target cells are retained on the column, while unlabeled cells flow through. For negative selection, biotinylated antibodies against non-target cells are used to bind and magnetically label the non-target cells; after the cell-bead suspension is loaded onto the column, the collected unbound fraction contains the negatively selected target cells. |
| Intended Use | Positive or negative magnetic selection of cells using biotinylated antibodies. |
| Principle / Technology | Biotinylated antibodies bind to target (or non-target) cells, and Anti-Biotin Nanobeads subsequently label the antibody-bound cells via the anti-biotin ligand. During column-based magnetic separation in a separation magnet, magnetically labeled cells are retained in the column while unlabeled cells flow through, enabling positive or negative selection. |
| Sample Type | Cells labeled with biotinylated antibodies |
| Performance Range / Specifications | Matrix: dextran nanobeads Ligand: Anti-Biotin antibody Particle size: 50-100 nm Storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Specificity | Specifically binds biotinylated antibodies through the anti-biotin ligand. |
| Reaction Conditions / Protocol | Positive selection: 1) Incubate the cells with a biotinylated antibody so that the antibody binds the target cells. 2) Add Anti-Biotin Nanobeads to magnetically label the cells. 3) Load the cell-bead suspension onto an MS column or LS column placed in the separation magnet of a magnetic separation system. 4) Magnetically labeled target cells are retained on the column; unlabeled cells flow through. 5) Remove the column from the magnet and elute the target cells. Negative selection: 1) Incubate the cells with biotinylated antibodies against non-target cells. 2) Magnetically label with Anti-Biotin Nanobeads. 3) Load the cell-bead suspension onto the separation column in the magnet. 4) Collect the unbound flow-through fraction, which contains the negatively selected target cells. |
| Components / Formulation | Anti-Biotin Nanobeads (dextran magnetic nanobeads conjugated with anti-biotin antibody) supplied in storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Storage Conditions | Store at 2-8 °C protected from light. |
| Shelf Life | 6 months |
| Package Specifications | 0.1 mL 0.5 mL 1 mL |
| Product Form | Dextran magnetic nanobead suspension (50-100 nm) |
| Quality Control | When used in a mouse CD3ε sorting kit to isolate CD3ε+ T cells from C57BL/6 mouse spleen cells, the purity, recovery, and viability of the sorted cells are comparable to those obtained with mainstream commercial magnetic bead kits. |
| pH Range / Optimal pH | pH 7.2-7.4 (storage buffer) |
| Expiration Date / Stability | Valid for 6 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with MS columns, LS columns, and other suitable separation columns used with the separation magnets of mainstream magnetic separation systems. |
| Recommended Buffer System | Storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Application Notes / Precautions | Store protected from light. Use appropriate separation columns (e.g., MS or LS columns) together with a suitable magnetic separator. |
| Recognition Site | Biotin (binds biotinylated antibodies) |
For research use only, not for clinical use.
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