| Product Name | Annexin V-EGFP Apoptosis Detection Reagent |
| Catalog No. | CCAT-HMM-0025 |
| Description | Annexins are a class of calcium-dependent phospholipid-binding proteins widely distributed in the cytoplasm of eukaryotic cells, involved in intracellular signal transduction. However, only Annexin V has been reported to regulate the activity of certain PKCs. Annexin V selectively binds to phosphatidylserine (PS). PS is primarily distributed on the inner side of the cell membrane, adjacent to the cytoplasm. During the early stages of apoptosis, various cell types translocate phosphatidylserine to the cell surface, i.e., the outer side of the cell membrane. Once exposed to the cell surface, phosphatidylserine promotes coagulation and inflammatory responses. However, when Annexin V binds to phosphatidylserine that has been inverted to the cell surface, it can block the procoagulant and proinflammatory activities of phosphatidylserine. Annexin V labeled with the green fluorescent probe EGFP, i.e., Annexin V-EGFP, can be used to detect the outward flipping of phosphatidylserine, an important feature of apoptosis, in a simple and direct manner using flow cytometry or fluorescence microscopy. |
| Application | This product uses EGFP-labeled recombinant human Annexin V to detect phosphatidylserine that appears on the cell membrane surface during apoptosis. |
| Applicable Instruments | Flow cytometer, fluorescence microscope, or other fluorescence detection equipment |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; sterile ddH₂O |
| Storage | Store at 4°C away from light. |
| Shelf Life | 6 months |
| Notes | Testing has shown that repeated freeze-thaw cycles (5 times) do not significantly affect the detection performance of Annexin V-EGFP. However, to achieve optimal results, it is recommended to store the product at 4°C within 3–6 months and avoid repeated freeze-thaw cycles whenever possible. Bacterial or fungal contamination can severely impair detection performance. It is advisable to perform detection promptly after staining, as prolonged delays may lead to an increase in the number of apoptotic or necrotic cells. If trypsin was used during cell collection, care must be taken to remove any residual trypsin. Residual trypsin can digest and degrade Annexin V-EGFP, ultimately resulting in staining failure. Fluorescent substances are prone to quenching. When performing fluorescence observations, minimize observation time and ensure light-protected storage during handling and storage. For your safety and health, please wear a lab coat and disposable gloves during handling. |
For research use only, not for clinical use.
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