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| Product Name | Animal Tissue Nuclei Extraction Kit (density-gradient, ATAC and CUT&Tag) |
| Catalog No. | REKI-0081 |
| Description | A complete kit for extracting intact nuclei from animal tissue, pairing homogenisation or liquid-nitrogen grinding with density gradient centrifugation to strip away cytoplasmic and organelle contamination. Input is 15 mg of tissue per preparation with a homogeniser, or 40 mg with grinding, and purified nuclei verify by fluorescence or trypan blue staining under the microscope before chromatin assays. The output feeds directly into ATAC accessibility profiling and CUT&Tag interaction mapping with reduced organelle carryover. |
| Intended Use | Nuclei preparation from animal tissue for ATAC chromatin accessibility assays and CUT&Tag protein-DNA interaction studies. |
| Principle / Technology | Mechanical disruption releases nuclei which are then purified by density gradient centrifugation to remove debris and organelles. |
| Sample Type | Animal tissue |
| Performance Range / Specifications | 15 mg tissue input (homogeniser) or 40 mg (grinding); reduced organelle residue; nuclei quality checkable by staining. |
| Specificity | Density-gradient based purification protects nuclear integrity. |
| Reaction Conditions / Protocol | Disrupt tissue in the supplied buffer, purify nuclei by gradient centrifugation, verify by staining and proceed to chromatin assays. |
| Components / Formulation | Concentrated nuclei isolation buffer, purification buffer modules. |
| Storage Conditions | 2 to 8 °C |
| Package Specifications | 4 T, 12 T |
| Product Form | Liquid buffer kit |
| Key Features | Stain-verified nuclei output; low organelle carryover; straightforward single-day preparation. |
For research use only, not for clinical use.
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