Animal rRNA Depletion Kit

Name: Animal rRNA Depletion Kit
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Cat.No: NG-QCY-0017 Datasheet

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Product Details Related Products

Product Name	Animal rRNA Depletion Kit
Catalog No.	NG-QCY-0017
Description	A mixed-species RNase H depletion kit for removing rRNA from total RNA of model animals, mammals, reptiles, birds and aquatic species.
Intended Use	mRNA and noncoding RNA analysis across diverse animal species.
Principle / Technology	Animal-targeted probes hybridize to rRNA, followed by RNase H digestion and DNase I treatment to preserve mRNA and other noncoding RNA.
Detection Method	Downstream RNA-seq library preparation and sequencing.
Sample Type	Animal total RNA from model species, mammals, reptiles, birds and aquatic organisms.
Performance Range / Specifications	Input range 0.01–1 µg total RNA; 6, 24 or 96 reactions; approximately 1-hour workflow.
Sensitivity / LOD	Reported residual rRNA below 5%.
Specificity	Targets major cytoplasmic and mitochondrial rRNA classes, including 5S, 5.8S, 18S, 28S, 12S and 16S rRNA.
Reaction Conditions / Protocol	Hybridize the sample with animal rRNA probes, perform RNase H and DNase I digestion, and purify the rRNA-depleted RNA.
Components / Formulation	Mixed animal rRNA depletion probes, RNase H digestion reagents and DNase I treatment reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	6 reactions; 24 reactions; 96 reactions.
Product Form	Multi-component animal rRNA depletion kit.
Quality Control	Residual rRNA, species coverage and downstream RNA-seq performance are evaluated.
Key Features	Four-group animal coverage; below 5% residual rRNA; approximately 1-hour workflow; broad species compatibility.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Suitable for coding and noncoding RNA sequencing workflows.
Application Notes / Precautions	Use the depleted RNA promptly or store it at −80 to −65°C because low-concentration RNA is susceptible to degradation.

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For research use only, not for clinical use.