| Product Name | Alkaline Protease (AKP) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0006 |
| Description | Alkaline protease (AKP) belongs to the serine protease class of endopeptidases. In an alkaline environment, it acts on peptide bonds to hydrolyze proteins into amino acids, peptides, and free amino acids. It can also hydrolyze ester bonds and amide bonds, and possesses transesterification and transpeptidation functions. It has widespread applications in the enzyme detergent industry, food processing, brewing, pharmaceuticals, and leather processing. |
| Application | Alkaline protease can catalyze the hydrolysis of casein to produce tyrosine under alkaline conditions. Tyrosine can reduce phosphomolybdate compounds to form tungsten blue under alkaline conditions. The product has a characteristic absorption peak at 680 nm, and the activity of alkaline protease can be characterized by changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 50 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (50 Samples) |
| Detection Method | Folin method |
| Spectral Parameters | 680 nm |
| Signal Response | Incremental |
| Standard | Tyrosine |
| Reference Standards | y=0.8108x+0.0072 (R2=0.9999) |
| Standard Linear Range | 0.05-1.2 μmol/mL |
For research use only, not for clinical use.
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