- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Alkaline Phosphatase (ALP) Activity Assay Kit (pNPP Substrate) |
| Catalog No. | ETR-HMM-0075 |
| Description | A colorimetric assay kit for the quantitative measurement of alkaline phosphatase activity using para-nitrophenyl phosphate as the chromogenic substrate. Widely used in clinical chemistry, ELISA applications, and enzyme characterization studies. |
| Intended Use | Quantitative measurement of ALP activity in serum, plasma, cell lysates, and purified enzyme preparations for clinical diagnostic and research applications. |
| Principle / Technology | ALP catalyzes the hydrolysis of p-nitrophenyl phosphate to p-nitrophenol and inorganic phosphate under alkaline conditions (pH 9.8–10.5); p-nitrophenol exhibits strong yellow color with absorbance at 405 nm. |
| Detection Method | Colorimetric measurement at 405 nm using a microplate reader or spectrophotometer |
| Sample Type | Human serum, plasma, cell and tissue lysates, purified enzyme preparations |
| Performance Range / Specifications | Linear detection range: 0.1–500 U/L ALP activity; typical reaction time: 10–30 minutes at 37°C |
| Sensitivity / LOD | ≤0.05 U/L minimum detectable ALP activity |
| Specificity | Specific for alkaline phosphatase isoenzymes (tissue-nonspecific, intestinal, placental, and germ cell ALP); acid phosphatase does not react under alkaline assay conditions |
| Reaction Conditions / Protocol | Add 20 μL of sample to 200 μL of pre-warmed pNPP substrate solution (37°C), incubate at 37°C for 10 minutes, monitor absorbance at 405 nm, or stop reaction with NaOH and read endpoint absorbance |
| Components / Formulation | pNPP substrate tablets (5 mg each), diethanolamine buffer (1 M, pH 9.8) with 0.5 mM MgCl2, ALP positive control, stop solution (2 M NaOH) |
| Storage Conditions | -20°C for pNPP tablets (protect from moisture); diethanolamine buffer at 2–8°C |
| Shelf Life | 24 months for unopened components |
| Package Specifications | 1000 tests in 96-well plate format (200 μL reaction volume) |
| Product Form | Tablet-form substrate with liquid buffer |
| Quality Control | Each lot validated with purified calf intestinal ALP standard; substrate lot tested for spontaneous hydrolysis rate; linearity verified |
| Key Features | Tablet format ensures long-term substrate stability; simple one-step reaction protocol; directly compatible with standard ELISA readers at 405 nm; widely recognized and cited pNPP method |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
