- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | AEBSF Hydrochloride, Water-Soluble Serine Protease Inhibitor |
| Catalog No. | RERE-0034 |
| Description | AEBSF, the hydrochloride salt of 4-(2-aminoethyl)benzenesulfonyl fluoride, is a water-soluble, irreversible serine protease inhibitor with broad activity against trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It serves as a safer replacement for PMSF and DFP: its inhibitory constants are comparable, it dissolves readily in water, its aqueous solutions are more stable, and its oral LD50 in mice is higher than that of either predecessor. Reported biological effects include extended survival in models of acute parasite infection and suppression of TNF-alpha-triggered apoptotic signatures in a human gastric adenocarcinoma cell line. |
| Intended Use | Protease inhibition during protein extraction and purification; protection of proteins in cell culture at concentrations up to 0.25 mM |
| Principle / Technology | Irreversible sulfonylation of the active-site serine residue, permanently blocking protease function |
| Detection Method | Trypsin, chymotrypsin, plasmin, kallikrein and thrombin |
| Sample Type | Cell and tissue lysates; culture supernatants and purification intermediates |
| Performance Range / Specifications | Effective at 0.1 to 1 mM; inhibitory constants comparable to PMSF and DFP |
| Specificity | Broad-spectrum serine protease inhibition with negligible effect on other protease classes |
| Reaction Conditions / Protocol | Dissolve in water or DMSO at 50 mg per mL; add to extraction buffers at 0.1 to 1 mM final concentration |
| Components / Formulation | AEBSF hydrochloride crystals, 50 mg per vial |
| Storage Conditions | Powder at 4°C in dry conditions or at -20°C for long-term keeping; 36 months as supplied |
| Shelf Life | 36 months |
| Package Specifications | 50 mg |
| Product Form | White or greyish-white crystalline powder |
| Quality Control | Purity at least 98% confirmed on the lot record |
| Key Features | Water-soluble replacement for PMSF; lower toxicity; more stable aqueous solutions; suitable for live-cell protection work |
| Purity | At least 98% |
| Concentration | Dissolves in water or DMSO at 50 mg per mL |
| Activity / Unit Definition | Irreversible serine protease inhibition |
| Molecular Weight | 239.7 |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Wet ice |
| Expiration Date / Stability | 36 months as powder; aqueous solutions noticeably more stable than those of PMSF |
| Regulatory / Compliance | For research use only |
| Compatibility | Compatible with extraction, cell culture and downstream purification workflows |
| Application Notes / Precautions | Sulfonyl fluoride; handle with gloves and eye protection; published work notes biological effects beyond protease inhibition, so interpret assay readouts accordingly |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction, purification intermediates protected from degradation, cell culture supernatant analysis |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
