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Acyl-CoA Synthetase (ACS)

Cat.No: IEC-HMM-0124 Datasheet

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Product Name Acyl-CoA Synthetase (ACS)
Catalog No. IEC-HMM-0124
Description Alta DiagnoTech Acyl-CoA Synthetase (ACS) is a recombinant enzyme supplied as white lyophilized powder. The enzyme has a molecular weight of 77 kDa (SDS-PAGE) by SDS-PAGE.
Intended Use For free fatty acid determination in blood.
Principle / Technology One unit converts 1 µmol of fatty acid to acyl-CoA per minute at pH 7.5 and 37°C.
Sample Type Blood samples in free fatty acid assay workflows
Performance Range / Specifications EC 6.2.1.3; CAS 9013-18-7; Specific activity: ≥5 U/mg protein
Reaction Conditions / Protocol Optimum pH: 7.5; Optimum temperature: 50°C; pH stability: pH 6.5-7.5 at 25°C for 18 hours; Thermal stability: Below 45°C at pH 7.5 for 10 minutes; Inhibitors: Ag+, Hg2+, Fe3+, Zn2+, and Cu2+
Components / Formulation White lyophilized powder
Storage Conditions Store at or below -20°C.
Shelf Life 18 months at -20°C.
Product Form White lyophilized powder
Key Features White lyophilized powder; Molecular weight: 77 kDa (SDS-PAGE); EC 6.2.1.3; Specific activity: ≥5 U/mg protein; Optimum pH: 7.5
Activity / Unit Definition Specific activity: ≥5 U/mg protein; Unit definition: One unit converts 1 µmol of fatty acid to acyl-CoA per minute at pH 7.5 and 37°C.
Molecular Weight 77 kDa (SDS-PAGE)
Source / Origin Recombinant
pH Range / Optimal pH 7.5
Expiration Date / Stability pH 6.5-7.5 at 25°C for 18 hours; Below 45°C at pH 7.5 for 10 minutes
Alternative Names / Synonyms Acyl-CoA Synthetase (ACS); ACS

For research use only, not for clinical use.

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