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| Product Name | Acyl-CoA Synthetase (ACS) |
| Catalog No. | IEC-HMM-0124 |
| Description | Alta DiagnoTech Acyl-CoA Synthetase (ACS) is a recombinant enzyme supplied as white lyophilized powder. The enzyme has a molecular weight of 77 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | For free fatty acid determination in blood. |
| Principle / Technology | One unit converts 1 µmol of fatty acid to acyl-CoA per minute at pH 7.5 and 37°C. |
| Sample Type | Blood samples in free fatty acid assay workflows |
| Performance Range / Specifications | EC 6.2.1.3; CAS 9013-18-7; Specific activity: ≥5 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 7.5; Optimum temperature: 50°C; pH stability: pH 6.5-7.5 at 25°C for 18 hours; Thermal stability: Below 45°C at pH 7.5 for 10 minutes; Inhibitors: Ag+, Hg2+, Fe3+, Zn2+, and Cu2+ |
| Components / Formulation | White lyophilized powder |
| Storage Conditions | Store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | White lyophilized powder |
| Key Features | White lyophilized powder; Molecular weight: 77 kDa (SDS-PAGE); EC 6.2.1.3; Specific activity: ≥5 U/mg protein; Optimum pH: 7.5 |
| Activity / Unit Definition | Specific activity: ≥5 U/mg protein; Unit definition: One unit converts 1 µmol of fatty acid to acyl-CoA per minute at pH 7.5 and 37°C. |
| Molecular Weight | 77 kDa (SDS-PAGE) |
| Source / Origin | Recombinant |
| pH Range / Optimal pH | 7.5 |
| Expiration Date / Stability | pH 6.5-7.5 at 25°C for 18 hours; Below 45°C at pH 7.5 for 10 minutes |
| Alternative Names / Synonyms | Acyl-CoA Synthetase (ACS); ACS |
For research use only, not for clinical use.
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